Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    In vitro Anticholinesterase and Neurotoxicity Activities of Ocotea aciphylla Fractions

    Monique Marylin A. de A. Carneiro2,3, Rodrigo Souza Conceição2, Isabella Mary Alves Reis4, Alessandra Bispo Da Silva3, Joana Da Luz Oliveira3, Alexsandro Branco4, Silvia Lima Costa3, Mariana Borges Botura2★★ Corresponding author

    1. 1Department of Health, Laboratory of Toxicology, Brazil.
    2. 2Department of Biochemistry and Biophysics, Institute of Health Sciences, Laboratory of Neurochemistry and Cell Biology, Federal University of Bahia, Salvador, Bahia, Brazil.
    3. 3Department of Health, Laboratory of Phytochemistry, State University of Feira de Santana, Feira de Santana, Brazil.

    CORRESPONDENCE

    Mariana Borges Botura

    Department of Biochemistry and Biophysics, Institute of Health Sciences, Laboratory of Neurochemistry and Cell Biology, Federal University of Bahia, Salvador, Bahia, Brazil.

    mbbotura@uefs.br

    Received: 18-08-2017; Revised: 19-10-2017.

    Volume 14, Issue 57s · pp. S448–S452 · PUBLISHED 10 September 2018 · DOI: 10.4103/pm.pm_366_17

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Ocotea species are known to produce secondary metabolites with a range of biological activities. This study aimed to evaluate the in vitro acetylcholinesterase (AChE) inhibition and neurotoxicity activities of the Ocotea aciphylla leaves. Materials and Methods: The in vitro anticholinesterase effect of crude extracts of O. aciphylla was investigated by means of spectrophotometric microplate assay. The most active extract, aqueous extract (AQE), was fractionated using column chromatography with silica gel as stationary phase to furnish several fractions that were also evaluated for the anticholinesterase effect. The neurotoxicity activity of AQE and active fraction (F9) was investigated in rat adrenal medulla pheochromocytoma strain cultures by 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetrazolium bromide test. The chemical characterization of the most active fraction was performed through high‑performance liquid chromatography coupled with mass spectrometry multistage (HPLC‑MS/MS). Results: Ethanolic extract (EE) and AQE exhibited significant inhibitory effects of the activity of AChE, with inhibitory concentration (IC50) of 443.7 and 412.8 µg/mL, respectively. Among the fractions, F9 was more effective AChE inhibition with IC50 of 286.2 µg/mL. In the neurocytotoxicity assays, only the F9, at the highest concentration (500 µg/mL), induced a significant reduction in cell viability. HPLC‑MS/MS analysis of the active fraction enabled the characterization of the natural compounds, i.e., procyanidin B‑type dimer, propelargonidin dimer, catechin, and methoxy‑luteolin‑deoxyhexose‑hexose. Conclusion: The leaves of O. aciphylla showed in vitro anticholinesterase activity and low neurotoxicity, and these effects might be related to the presence of phenolic compounds.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Carneiro, M. M. A. D. A., Conceição, R. S., Reis, I. M. A., Silva, A. B. D., Oliveira, J. D. L., Branco, A., Costa, S. L., & Botura, M. B. (2018). In vitro Anticholinesterase and Neurotoxicity Activities of Ocotea aciphylla Fractions. Pharmacognosy Magazine, 14(57s), S448–S452. https://doi.org/10.4103/pm.pm_366_17