Antithyroidic and Hepatoprotective Properties of High‑Resolution Liquid Chromatography–Mass Spectroscopy‑Standardized Piper betle Leaf Extract in Rats and Analysis of Its Main Bioactive Constituents

ABSTRACT

Background: Hyperthyroidism can be a serious health problem, if not treated properly. This investigation primarily aimed to evaluate the thyroid regulatory and hepatoprotective activities of ethyl acetate fraction (EPBL) of Piper betle leaf extract in L‑thyroxine (L‑T4)‑induced hyperthyroid rats. Materials and Methods: Effects of EPBL extract at a prestandardized dose of 50 mg/kg (p. o.) were studied in L‑T4 (500 μg/kg/d, i. p.)‑administered rats to examine the alterations in the levels of serum triiodothyronine (T3), thyroxine (T4), thyrotropin, alanine transaminase, and aspartate aminotransferase; in the activities of hepatic 5’‑monodeiodinase (5’DI), glucose‑6‑phosphatase (G‑6‑Pase), and Na+‑K+‑ATPase; in the level of tissue malondialdehyde (MDA) and lipid hydroperoxides (LOOHs); and in the activities of antioxidants. Results: Administration of the EPBL extract reversed the T4‑induced increase in serum thyroid hormones, the liver marker enzymes, MDA, and LOOH but enhanced the activities of antioxidative enzymes and reduced the glutathione content. Light microscopic findings of liver histology revealed distorted hepatic tissue architecture in hyperthyroid animals that were improved by the EPBL administration. High‑resolution liquid chromatography–mass spectroscopy analysis revealed four main flavonoid glycosides such as quercetin, rutin, kaempferol, and luteolin. Conclusion: For the first time, our findings revealed the antithyroidic property of EPBL in T4‑induced hyperthyroidism, without any hepatotoxicity. The antithyroidic and antioxidative properties of EPBL in hyperthyroid animals could be due to the presence of flavonoid glycosides in the extract which might have inhibited the thyroid hormone secretion and conversion of T4 to T3 through an inhibition of 5’DI.

KEYWORDS

• Antioxidants
• high‑resolution liquid chromatography–mass spectroscopy
• hyperthyroidism
• lipid peroxidation
• Piper betle

Showing references and in-text citations exactly as published.

• DOI10.4103/pm.pm_450_18Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Mag..

• Article · VOLUME 14, ISSUE 59SCharacterization of the Secondary Metabolites from Endophytic Fungi Nodulisporium sp. Isolated from the Medicinal Plant Mikania laevigata (Asteraceae) by Reversed‑Phase High‑Performance Liquid Chromatography Coupled with Mass Spectrometric MultistageIsabella M. A. Reis, Fernanda P. C. Ribeiro, Paulo R. M. Almeida, Larissa C. B. Costa, Hélio M. Kamida, Ana P. T. Uetanabaro, Alexsandro Branco · pp. S495–S498Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 59SNeuropharmacological Effects of Methanolic Extract of Clerodendrum viscosum Leaves on Wistar Albino RatsMeera Rath, Ayon Bhattacharya, Soumya Santra, Karmajeet Rath, Goutam Ghosh, Bijaya B. Nanda · pp. S507–S514Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 59SInducible Nitric Oxide Synthase Downregulation and Apoptotic Activity of Pleurotus florida (Oyster Mushroom)Aathishree Vanji Paramasivan, Ramaraj Thirugnanasampandan, Vasantharani Selvaraj, Bhuvaneswari Gunasekaran, Madhusudhanan Gogul Ramnath, Shanmugam Krishnakumari, Subramanian Kathiravan · pp. S515–S519Original ArticleSame type: Article
• Article · VOLUME 14, ISSUE 59SElucidation of the Molecular Mechanism of Tempol in Pentylenetetrazol‑induced Epilepsy in Mice: Role of Gamma‑aminobutyric Acid, Tumor Necrosis Factor‑alpha, Interleukin‑1β and C‑FosLeguo Zhang, Tao Wu, Amit Kandhare, Anwesha Mukherjee, Gang Guo, Subhash L. Bodhankar · pp. S520–S527Original ArticleSame type: Article