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    Study on Pharmacokinetics and Tissues Distribution of Neomangiferin, Mangiferin, Timosaponin BII, Timosaponin BIII, and Timosaponin AIII after Oral Administration of Anemarrhenae Rhizoma Extract in Rats

    De Ji2,3, Jin‑Chun Qiu4, Xiao‑Nan Su2, Yu‑Wen Qin5, Min Hao2, Lin Li2, Tu‑Lin Lu2, Xiao‑Kun Li3,6, Cheng‑Xi Jiang5,6★★ Corresponding author

    1. 1Department of Chinese Medicinal Processing, School of Pharmacy, Nanjing University of Chinese Medicine.
    2. 2Molecular Pharmacology Research Center, School of Pharmaceutical Science, Wenzhou Medical University.
    3. 3Department of Pharmacy, Children’s Hospital of Nanjing Medical University, Nanjing.
    4. 4Department of Chinese Medicinal Resources, Life Sciences Institute, Wenzhou University.
    5. 5Department of Chinese Medicinal Resources, Biomedical Collaborative Innovation Center of Zhejiang, Wenzhou, PR China, School of Pharmacy, Nanjing University of Chinese Medicine.

    CORRESPONDENCE

    Cheng‑Xi Jiang

    Department of Chinese Medicinal Resources, Biomedical Collaborative Innovation Center of Zhejiang, Wenzhou, PR China, School of Pharmacy, Nanjing University of Chinese Medicine.

    chengxiwmu@126.com

    Received: 06-02-2019; Revised: 12-03-2019.

    Volume 15, Issue 65 · pp. 557–567 · PUBLISHED 19 September 2019 · DOI: 10.4103/pm.pm_65_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: The main objective of the study is to establish an ultra‑high‑performance liquid chromatography‑tandem mass spectrometry (MS/MS) method to determine the concentrations of five bioactive constituents in rats’ plasma and various tissues. Materials and Methods: The analytes were separated on a C18 reversed‑phase column. A triple‑quadrupole MS/MS equipped with an electrospray ionization source was used as a detector. The main pharmacokinetic parameters were estimated with Drug and Statistics 2.0 Software Package. Results: Neomangiferin and mangiferin exhibit poor oral absorption and slow clearance from the body. Timosaponin BII and timosaponin BIII could be quickly absorbed into the blood circulation and showed double plasma concentration peaks. Timosaponin AIII exhibited a single peak in the plasma concentration‑time plot and pharmacokinetic parameters of timosaponin AIII indicated slower absorption, longer body residence time, and slower elimination than timosaponin BII and timosaponin BIII. The five analytes were widely distributed to most of the tissues. Neomangiferin and mangiferin exhibited the maximum concentration in the lung at 6 h after oral administration, the highest levels of timosaponin BII and timosaponin BIII were also observed in the lung at 1 h after oral administration, and the maximum concentration of timosaponin AIII was observed in the liver. Conclusion: The findings of the present study might be helpful to better understand the pharmacokinetics and distribution of AR bioactive constituents in vivo, which would facilitate the clinical application of AR.

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      Ji, D., Qiu, J., Su, X., Qin, Y., Hao, M., Li, L., Lu, T., Li, X., & Jiang, C. (2019). Study on Pharmacokinetics and Tissues Distribution of Neomangiferin, Mangiferin, Timosaponin BII, Timosaponin BIII, and Timosaponin AIII after Oral Administration of Anemarrhenae Rhizoma Extract in Rats. Pharmacognosy Magazine, 15(65), 557–567. https://doi.org/10.4103/pm.pm_65_19