Structural Characterization and Immune Regulation of a New Heteropolysaccharide from Catathelasma imperiale (Fr.) Sing
Lu Liu2, Xiang Ding2,3, Yiling Hou2★★ Corresponding author
- 1Key Laboratory of Southwest China Wildlife Resources Conservation (Ministry of Education), College of Life Sciences, China West Normal University, China.
- 2College of Environmental Science and Engineering, China West Normal University, Nanchong, Sichuan Province, China.
CORRESPONDENCE
Yiling Hou
College of Environmental Science and Engineering, China West Normal University, Nanchong, Sichuan Province, China.
Received: 03-01-2019; Revised: 12-02-2019.
Volume 15, Issue 65 · pp. 621–630 · PUBLISHED 19 September 2019 · DOI: 10.4103/pm.pm_673_18
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ABSTRACT
Background: Polysaccharide has played the part of great role in pharmacology and physiology. Materials and Methods: In this study, the polysaccharides (CIS‑A) from Catathelasma imperiale (Fr.) Sing, were isolated and purified by hot water extraction technology and column chromatography, respectively. Chemical methods, infrared spectrum, high‑performance gel‑permeation chromatography, high‑performance liquid chromatography, gas chromatography–mass spectrometry, 1H nuclear magnetic resonance spectroscopy (NMR), 13C NMR, and two‑dimensional NMR were used to characterize the polysaccharides of CIS‑A. The anticancer and immunomodulatory ability of the polysaccharides (CIS‑A) from the fruiting body of C. imperiale (Fr.) Sing was also investigated. Results: The structural feature analysis showed the polysaccharide (CIS‑A) which had a molecular weight of 50486 Da was mainly composed of α‑D‑glucose pyranose (α‑D‑Glcp) and β‑L‑fucose pyranose (β‑L‑Fucp). It had a backbone of three 1, 3‑linked α‑D‑Glcp. There is a branch at the C2 of the polysaccharide backbone. The branches were mainly composed of two 2, 3‑linked β‑L‑Fucp residue. Antitumor activity results showed that CIS‑A could inhibit the growth of S180 tumor and promote the apoptosis of L929 cells. Immunoregulatory activity results showed that CIS‑A could promote the proliferation of T‑cells and promote B‑cells by affecting G0/G1 phase, S phase, and G2/M phase. It also could promote the proliferation and phagocytosis of macrophages and induce cytokine release. Conclusion: Polysaccharide CIS‑A can be used as a candidate drug for antitumor and immunomodulator.
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Liu, L., Ding, X., & Hou, Y. (2019). Structural Characterization and Immune Regulation of a New Heteropolysaccharide from Catathelasma imperiale (Fr.) Sing. Pharmacognosy Magazine, 15(65), 621–630. https://doi.org/10.4103/pm.pm_673_18