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    Article

    Antitumor Effects and Mechanism of Protein from Panax ginseng C. A. Meyer on Human Breast Cancer Cell Line MCF‑7

    Xilin Wan2,3, Xin Jin4, Yuhe Ren5, Yang Xiu2, Yu Li3, Changbao Chen2★, Shuying Liu2★★ Corresponding author

    1. 1Key Laboratory of Ginseng Chemistry and Pharmacology of Jilin Province, Jilin Ginseng Academy, Changchun University of Traditional Chinese Medicine, China.
    2. 2Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, China.
    3. 3Key Laboratory of Molecular Epigenetics of the Ministry of Education (MOE), Northeast Normal University, China.
    4. 4Institute of Special Animal and Plant Science, Chinese Academy of Agricultural Sciences, Changchun, Jilin, People’s Republic of, China.

    CORRESPONDENCE

    Changbao Chen

    Engineering Research Center of Chinese Ministry of Education for Edible and Medicinal Fungi, Jilin Agricultural University, China.

    ccb2021@126.com

    Received: 02-04-2019; Revised: 26-04-2019.

    Volume 15, Issue 65 · pp. 715–721 · PUBLISHED 19 September 2019 · DOI: 10.4103/pm.pm_151_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Aims: Protein components were extracted from ginseng and antitumor activity in human breast cancer cell line MCF‑7 was investigated. Settings and Design: Five methods were applied to extract GP and the antitumor effects and mechanism of GP on MCF‑7 were explored, including proliferation, cell cycle, apoptosis, and migration. Subjects and Methods: Five extraction methods were employed. MCF‑7 cell proliferation was measured using a cell counting kit‑8 with different GP concentrations (0, 0.25, 0.5, 1, 2, and 4 mg/mL) and half inhibitory concentration values were calculated. Cell cycle, morphology, and apoptosis were investigated using immunofluorescence staining and flow cytometry. Migration was probed by scratch wound healing and transwell assays. Quantitative‑polymerase chain reaction and western blotting were performed to analyze apoptosis‑associated gene/protein expression. Statistical Analysis: Experimental data were analyzed by Microsoft Excel and SPSS software. Results: Acetone extraction achieved the highest GP purity and yield. GP inhibited proliferation of MCF‑7 in a time‑dependent manner and induced cell cycle arrest at G1/S and apoptosis. Scratch wound healing and transwell assays showed that cell migration was also inhibited by GP and expression levels of Bcl‑2 and Bax were affected. Conclusion: GP elicits antitumor activity by inhibiting cell proliferation and migration and inducing cell cycle arrest and apoptosis in MCF‑7 cells and may act via the Bcl-2 and Bax apoptotic pathway.

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      Wan, X., Jin, X., Ren, Y., Xiu, Y., Li, Y., Chen, C., & Liu, S. (2019). Antitumor Effects and Mechanism of Protein from Panax ginseng C. A. Meyer on Human Breast Cancer Cell Line MCF‑7. Pharmacognosy Magazine, 15(65), 715–721. https://doi.org/10.4103/pm.pm_151_19