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    Article

    A Green Method for Preparation of Curcuminoid‑rich Curcuma longa Extract and Evaluation of its Anticancer Activity

    Likit Lateh2, Supreeya Yuenyongsawad2, Haixia Chen3, Pharkphoom Panichayupakaranant2,4★★ Corresponding author

    1. 1Department of Pharmacognosy and Pharmaceutical Botany, Faculty of Pharmaceutical Sciences, Prince of Songkla University.
    2. 2Tianjin Key Laboratory for Modern Drug Delivery and High‑Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.
    3. 3Phytomedicine and Pharmaceutical Biotechnology Excellence Center, Faculty of Pharmaceutical Sciences, Prince of Songkla University, Hat‑Yai, Songkhla, Thailand.

    CORRESPONDENCE

    Pharkphoom Panichayupakaranant

    Tianjin Key Laboratory for Modern Drug Delivery and High‑Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, China.

    pharkphoom.p@psu.ac.th

    Received: 05-04-2019; Revised: 29-05-2019.

    Volume 15, Issue 65 · pp. 730–735 · PUBLISHED 19 September 2019 · DOI: 10.4103/pm.pm_162_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: To establish a green method for preparation of curcuminoid‑rich C. longa extracts (CRE) using microwave‑assisted extraction (MAE) together with a simple one‑step fractionation and to investigate the anticancer activity of CRE compared with the three marker curcuminoids. Materials and Methods: MAE was used as a green extraction method, and a macroporous resin (Diaion® HP‑20) column was used for fractionation of C. longa extract to produce CRE. The sulforhodamine B assay was used to evaluate in vitro anticancer activity of the curcuminoids. Results: The optimal conditions of MAE for extraction of curcuminoids are employing ethanol as the solvent and using three irradiation cycles in a microwave powered at 900 W (one cycle is 3 min power‑on and 30 s power‑off). The curcuminoid extract was subsequently fractionated on a Diaion® HP‑20 column eluted with 55% and 60% v/v ethanol, respectively, to obtain CRE that contained total curcuminoids of 88% w/w. CRE exhibited good anticancer activities against A549, MCF‑7, HeLa, and HT‑29 cells, with 50% inhibitory concentration values of 5.2, 4.5, 7.5, and 8.3 µg/mL, respectively, which almost equals those of the marker curcuminoids. Conclusion: This study indicated a potential use of CRE for anticancer purposes in food and nutraceutical applications. CRE has more advantages than pure curcuminoids for industrial applications in terms of using simple, low‑cost, and environmentally friendly processes.

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      Lateh, L., Yuenyongsawad, S., Chen, H., & Panichayupakaranant, P. (2019). A Green Method for Preparation of Curcuminoid‑rich Curcuma longa Extract and Evaluation of its Anticancer Activity. Pharmacognosy Magazine, 15(65), 730–735. https://doi.org/10.4103/pm.pm_162_19