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    Inhibitory Effects of Fermented Soybean Tempeh on the Anti‑Adhesive Properties of Actinomyces viscosus and Plaque Growth in vitro

    Allimalar Sathiaseelan2, Chong Seng Shit2,3★, Tsun‑Thai Chai3,4★ Corresponding author

    1. 1Departments of, University Tunku Abdul Rahman, Perak, Malaysia.
    2. 2Biological Science and, Malaysia.
    3. 3Centre for Bio‑Diversity Research, University Tunku Abdul Rahman, Perak, Malaysia.
    4. 4Chemical Science, Faculty of Science, Universiti Tunku Abdul Rahman, Malaysia.

    CORRESPONDENCE

    Chong Seng Shit

    Biological Science and, Malaysia.

    scseng@utar.edu.my

    Received: 22-01-2019; Revised: 06-03-2019.

    Volume 15, Issue 66s · pp. S371–S376 · PUBLISHED 28 November 2019 · DOI: 10.4103/pm.pm_29_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: Here, we investigated the efficacy of tempeh HXF on other cariogenic virulence traits of A. viscosus such as adhesive properties, acid production, and plaque growth. Materials and Methods: Anti adhesion of HXF was assessed based on its effects on the number of cells adhering to the surface of tooth in sucrose‑dependent (SD) and sucrose‑independent (SI) medium. The potential of HXF to inhibit the capability of A. viscosus to generate acids was investigated by pH drop assay. The HXF at different concentrations were used to determine the LC50 based on brine shrimp lethality assay. Finally, the prospect of HXF as an inhibitor of plaque formation was investigated using artificial saliva‑coated denture as an in vitro batch model. Results: HXF significantly decreased colony‑forming unit of SD (1.07 log reduction) and SI (0.56 log reduction)‑mediated adsorption of bacterial cells onto the tooth surface over 4‑ and 12‑h incubation, respectively. Acid production was reduced after treated with HXF in a dose‑dependent manner. Finally, a substantial reduction in plaque coverage area >55% was found on the HXF treated‑denture. Conclusion: The anti‑biofilm effect of HXF was associated with the suppression of A. viscosus adhesion to tooth surfaces and reduction in acid production. Furthermore, in vitro anti‑plaque potential of HXF was demonstrated.

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      Sathiaseelan, A., Shit, C. S., & Chai, T. (2019). Inhibitory Effects of Fermented Soybean Tempeh on the Anti‑Adhesive Properties of Actinomyces viscosus and Plaque Growth in vitro. Pharmacognosy Magazine, 15(66s), S371–S376. https://doi.org/10.4103/pm.pm_29_19