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    Article

    Statistical Approach towards Optimization of Extraction Process of Karanjin from Pongamia pinnata Seeds

    Sartaj Akhtar Ansari2, Lubna Abidin2★, Tinku Gupta2, Mohd. Mujeeb2, Aftab Ahmad3, Varish Ahmad3, Showkat Rasool Mir4★ Corresponding author

    1. 1Department of Pharmacognosy and Phytochemistry, Phytomedicine Laboratory, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
    2. 2Department of Health Information Technology, Jeddah Community College, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.
    3. 3Department of Pharmacognosy and Phytochemistry, Phytopharmaceutical Research Laboratory, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.

    CORRESPONDENCE

    Lubna Abidin

    Department of Health Information Technology, Jeddah Community College, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.

    lubna.sch@jamiahamdard.ac.in

    Received: 03-12-2018; Revised: 19-06-2019.

    Volume 15, Issue 66s · pp. S419–S425 · PUBLISHED 28 November 2019 · DOI: 10.4103/pm.pm_609_18

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: Use of Box–Behnken design for optimization of extraction of karanjin from P. pinnata seeds. Materials and Methods: Design expert software was used for optimization purpose. Extraction temperature, extraction time and solvent‑to‑drug ratio were taken as input variables which affected the karanjin content. Quantification of karanjin in different extracts was done through high‑performance liquid chromatography using methanol and water (80:20% v/v) as mobile phase. Results: Ultrasound‑assisted extraction stood out to be the most efficient mode for extraction of karanjin using methanol as solvent. Extraction temperature of 57.85°C, extraction time of 25.45 min, and solvent‑to‑drug ratio of 86.4709% v/w were established as optimum conditions for extraction of karanjin from P. pinnata seeds. Under such extraction conditions, 8.33%w/w karanjin was extracted. Conclusion: From our study, it was concluded that non-thermal methods are a better choice for extraction of karanjin and methanol is the most efficient solvent for the same. All the three input variables significantly affected karanjin content which was confirmed by model fitting and analysis of regression coefficients. Our research shows the relevance of a statistical approach in phytocompound research area which makes the extraction process cheap and less laborious.

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      Ansari, S. A., Abidin, L., Gupta, T., Mujeeb, M., Ahmad, A., Ahmad, V., & Mir, S. R. (2019). Statistical Approach towards Optimization of Extraction Process of Karanjin from Pongamia pinnata Seeds. Pharmacognosy Magazine, 15(66s), S419–S425. https://doi.org/10.4103/pm.pm_609_18