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    Evaluation of Lipotropic Effect of Herbal Formulation on Hepatic Fat Accumulation in Rats Fed with Methionine-Choline Deficient Diet

    Prasanna Raja Chandrasekaran1★, Sasikumar Murugan1, Edwin Jothie Richard1, Bharathi Bethapudi1, Divya Purusothaman1, Chandrasekaran Chinampudur Velusami1, Prashanth D’Souza2, Deepak Mundkinajeddu1, Muralidhar S. Talkad2★ Corresponding author

    1. 1Department of Biology, India.
    2. 2Department of Animal Health Science, R and D Centre, Natural Remedies Private Limited, Bengaluru, Karnataka, India.

    CORRESPONDENCE

    Prasanna Raja Chandrasekaran

    Department of Biology, India.

    prasanna.raja@naturalremedy.com

    Received: 15-03-2019; Revised: 29-05-2019.

    Volume 15, Issue 66s · pp. S476–S482 · PUBLISHED 28 November 2019 · DOI: 10.4103/pm.pm_111_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: To investigate the lipotropic activity of the proprietary herbal formulation (PHF) containing Acacia nilotica and Curcuma longa. Materials and Methods: Fatty liver disease was induced in Wistar rats by feeding methionine/choline‑deficient (MCD) diet for 4 weeks. Animals were concurrently treated with PHF (at 50, 100, 200, and 400 mg/kg rat body weight/day) for 4 weeks. Methionine/choline‑sufficient (MCS) diet‑fed rats were used as control. Serum biochemistry and liver parameters were determined at the end of experimental period. Further, anti‑lipogenic and lipolytic activity of PHF extract was studied in HepG2 cells. Results: Rats fed with MCD diet, showed significant increase in liver lipids, triglycerides, cholesterol, thiobarbituric acid reactive substance, and serum alanine transaminase (ALT) and decreased serum triglyceride level compared to MCS diet‑fed rats indicating significant fat accumulation and liver damage. PHF treatment significantly decreased the liver lipids, triglyceride and serum ALT compared to MCD diet‑fed rat. Histological evaluation revealed the restoration of hepatic architecture after PHF treatment. In in vitro studies, the PHF extract decreased the oleic acid induced fat accumulation in HepG2 cells. Conclusion: The study demonstrated the lipotropic effect of PHF evident from decreased fat accumulation and antilipogenic activity. These data suggests that PHF could be a potential supplement for preventing fatty liver.

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      Chandrasekaran, P. R., Murugan, S., Richard, E. J., Bethapudi, B., Purusothaman, D., Velusami, C. C., D’Souza, P., Mundkinajeddu, D., & Talkad, M. S. (2019). Evaluation of Lipotropic Effect of Herbal Formulation on Hepatic Fat Accumulation in Rats Fed with Methionine-Choline Deficient Diet. Pharmacognosy Magazine, 15(66s), S476–S482. https://doi.org/10.4103/pm.pm_111_19