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    Article

    Embelin from Embelia ribes Ameliorates Oxidative Stress and Inflammation in High‑Fat Diet‑Fed Obese C57BL/6 Mice

    Priyanka Bansal1, Uma Bhandari1★, Sayeed Ahmad2★ Corresponding author

    1. 1Departments of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
    2. 2Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.

    CORRESPONDENCE

    Uma Bhandari

    Departments of Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.

    uma_bora@hotmail.com

    Received: 13-03-2020; Revised: 06-05-2020; Accepted: 06-07-2020.

    Volume 16, Issue 5S · pp. S443–S449 · PUBLISHED 30 November 2020 · DOI: 10.4103/pm.pm_77_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This study was designed to isolate and characterize embelin from Embelia ribes and further to assess its role in alleviating oxidative stress and chronic inflammation in the high‑fat diet (HFD) fed obese C57BL/6 mice. Materials and Methods: Embelin extracted from berries of E. ribes with n‑hexane by soxhlet extraction was characterized using high‑performance thin‑layer chromatography, infrared and liquid chromatography‑mass spectrometry analyses. The obesity was induced by feeding of HFD for 8 weeks. Embelin (50 mg/kg/day, p.o.) was administered from 5th to 8th weeks along with HFD. After 8 weeks, the body weight gain and body mass index were calculated. Then, animals were sacrificed; serum and tissues were collected to further assess the various biomarkers of oxidative stress and inflammation. Results: The presence of embelin was confirmed using the above‑mentioned analytical techniques. Treatment with Embelin showed amelioration of obesity biomarkers along with the substantial decline in levels of protein expression of nuclear factor erythroid 2‑related factor and nuclear factor kappa‑B in liver tissue. Treatment with embelin also normalizes the liver tissue levels of thiobarbituric acid reactive substances, glutathione, superoxide dismutase, and catalase. The histopathological analysis of liver tissue showed significant prevention of necrotic and inflammatory changes in embelin treated HFD fed mice. Conclusion: The results of the study clearly indicated the potential of embelin in ameliorating obesity by alleviating oxidative stress and inflammation induced by HFD in C57BL/6 mice.

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      Bansal, P., Bhandari, U., & Ahmad, S. (2020). Embelin from Embelia ribes Ameliorates Oxidative Stress and Inflammation in High‑Fat Diet‑Fed Obese C57BL/6 Mice. Pharmacognosy Magazine, 16(5S), S443–S449. https://doi.org/10.4103/pm.pm_77_20