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    DNA Barcoding Markers to Identify Intraspecies Genetic Variations in Three Ecotypes of Abrus precatorius L.

    Srinivasan Marimuthu1, G. P. Pratap2, Goli Penchala Prasad3★, Shyam Baboo Prasad4, Anupam Kumar Mangal5, N. Srikanth6★ Corresponding author

    1. 1Research Officer (Biochemistry), Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India.
    2. 2Survey of Medicinal Plants Unit, Central Research Institute for Unani Medicine, Hyderabad, Telangana, India.
    3. 3Assistant Director In-charge, Regional Ayurveda Institute for Fundamental Research, India.
    4. 4Research Officer (Pharmacognosy), Regional Ayurveda Institute for Fundamental Research, Pune, Maharashtra, India.
    5. 5Assistant Director (Pharmacognosy), Central Council for Research in Ayurvedic Sciences, India.
    6. 6Deputy Director General, Central Council for Research in Ayurvedic Sciences, New Delhi, India.

    CORRESPONDENCE

    Goli Penchala Prasad

    Assistant Director In-charge, Regional Ayurveda Institute for Fundamental Research, India.

    drpenchalaprasadgoli@gmail.com

    Received: 01-02-2020; Revised: 29-05-2020; Accepted: 06-07-2020.

    Volume 16, Issue 5S · pp. S540–S545 · PUBLISHED 30 November 2020 · DOI: 10.4103/pm.pm_23_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The present study aims to compare three different varieties of A. precatorius which includes the species of white, black, and red which are used to study DNA barcoding marker and phylogenetic analysis. Materials and Methods: Whole genomic isolated DNA from three varieties (white, black, and red) of A. precatorius leaves and subjected to analysis of the polymerase chain reaction of rbcL, maturase K, and internal transcribed spacer 4,5 using 0.8% agarose gel electrophoresis. Results: The DNA barcoding markers and next‑generation sequencing can identify the intraspecies genetic variations among these closely related plant varieties of A. precatorius of white, black, and red. Conclusion: The intraspecies genetic variations among these three varieties of A. precatorius white, black, and red are closely related with A. precatorius isolate TMP 144 Ribulose 1,5-bisphosphate carboxylase/oxygenase large subunit gene 99.81%.

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      Marimuthu, S., Pratap, G. P., Prasad, G. P., Prasad, S. B., Mangal, A. K., & Srikanth, N. (2020). DNA Barcoding Markers to Identify Intraspecies Genetic Variations in Three Ecotypes of Abrus precatorius L.. Pharmacognosy Magazine, 16(5S), S540–S545. https://doi.org/10.4103/pm.pm_23_20