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    Article

    Red Seaweed (Eucheuma cottonii) Extract Promotes Human Keratinocyte Migration via p38 Mitogen‑Activated Protein Kinase Phosphorylation

    In Wook Kim1★, So Yeon Kim1, Marianti Manggau2, Harfina Finanda Anwar1, Nyoun Soo Kwon1, Kwang Jin Baek1★ Corresponding author

    1. 1Hye‑Young Yun, Dong‑Seok Kim Department of Biochemistry, Chung‑Ang University College of Medicine, Dongjak‑gu, Seoul, Korea, Indonesia.
    2. 2Faculty of Pharmacy, Hasanuddin University, Makassar, Indonesia.

    CORRESPONDENCE

    In Wook Kim

    Hye‑Young Yun, Dong‑Seok Kim Department of Biochemistry, Chung‑Ang University College of Medicine, Dongjak‑gu, Seoul, Korea, Indonesia.

    ds_kim@cau.ac.kr

    Received: 21-05-2019; Revised: 01-07-2019.

    Volume 16, Issue 67 · pp. 192–196 · PUBLISHED 11 February 2020 · DOI: 10.4103/pm.pm_203_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This study was performed to investigate the effects of EC extract on human keratinocyte migration. Materials and Methods: Cell migration was assessed using the scratch wound assay. Western blot analysis was performed to investigate the EC extract‑induced signaling pathways. Results: EC extract promoted HaCaT keratinocyte migration in a concentration‑dependent manner. To investigate the mechanism of EC extract‑induced migration, we examined the migration‑related signaling pathways. Western blot analysis showed that the EC extract showed no changes in extracellular signal‑activated kinase (ERK) but showed slight Akt activation. In contrast, the EC extract strongly phosphorylated p38 mitogen‑activated protein kinase (MAPK) and the subsequent downstream molecule, cAMP response element‑binding protein (CREB). To examine the involvement of p38 MAPK pathway in EC extract‑induced migration, SB203580, a specific inhibitor of p38 MAPK, was used. The results showed that EC extract‑induced migration was abrogated by SB203580 pretreatment. In addition, SB203580 also blocked p38 MAPK and CREB activation. Conclusion: Taken together, these data suggest that EC extract promotes migration in human keratinocytes via p38 MAPK and CREB phosphorylation.

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      Kim, I. W., Kim, S. Y., Manggau, M., Anwar, H. F., Kwon, N. S., & Baek, K. J. (2020). Red Seaweed (Eucheuma cottonii) Extract Promotes Human Keratinocyte Migration via p38 Mitogen‑Activated Protein Kinase Phosphorylation. Pharmacognosy Magazine, 16(67), 192–196. https://doi.org/10.4103/pm.pm_203_19