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    Vanillin an Active Constituent from Vanilla Bean Induces Apoptosis and Inhibits Proliferation in Human Colorectal Adenocarcinoma Cell Line

    Yandong Xie1, Jingyu Zhang1, Cheng Wang1, Qinglin Fan1, Yiling Zhang2★★ Corresponding author

    1. 1Departments of Gastroenterology, Hospital, Fourth Military Medical University, Baqiao, Xi’an, Shaanxi Province, China.
    2. 2Oncology, Tangdu Hospital, Fourth Military Medical University, Baqiao, Xi’an, Shaanxi Province, China.

    CORRESPONDENCE

    Yiling Zhang

    Oncology, Tangdu Hospital, Fourth Military Medical University, Baqiao, Xi’an, Shaanxi Province, China.

    zhangyilingtd@sina.com

    Received: 01-06-2019; Revised: 14-08-2019.

    Volume 16, Issue 67 · pp. 197–200 · PUBLISHED 11 February 2020 · DOI: 10.4103/pm.pm_235_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Aim: The aim of the study was to investigate the antiproliferative and proapoptotic effects of vanillin (VL) an active constituent from Vanilla bean in colon cancer (human colorectal adenocarcinoma cell line [HT-29]) cells. Methodology: We demonstrated the cell growth and apoptosis in HT-29 cells. The apoptotic measures were analyzed by analyzing mitochondrial membrane potential (Δψm) level; apoptotic bodies by acridine orange/ethidium bromide and Hoechst staining. Results: Our results indicated that VL induces apoptosis as evidenced by cell viability loss, resulting in the altered Δψm in HT-29 cells. Further, the prooxidant role of VL inhibit B-cell lymphoma 2 (Bcl2) expression with the simultaneous upregulation of BCL-2 associated X, Cytochrome c, Capspase-9 and 3 protein expressions in HT-29 cells. Conclusion: Thus, in this regard, we have concluded that VL induces apoptosis in HT-29 cells through inducing oxidative damage and modulates apoptotic marker expressions. VL may therefore be used as a therapeutic agent for colon cancer treatment.

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      Xie, Y., Zhang, J., Wang, C., Fan, Q., & Zhang, Y. (2020). Vanillin an Active Constituent from Vanilla Bean Induces Apoptosis and Inhibits Proliferation in Human Colorectal Adenocarcinoma Cell Line. Pharmacognosy Magazine, 16(67), 197–200. https://doi.org/10.4103/pm.pm_235_19