Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Computer-Aided Box–Behnken Outlook Towards Optimization of Extraction of Lawsone from Mehendi Leaves

    Lubna Abidin2, Mohd. Mujeeb2★, Mohd. Aqil3, Abul Kalam Najmi4, Aftab Ahmad5★ Corresponding author

    1. 1Phytomedicine Laboratory, Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education and Research, Departments of.
    2. 2Pharmaceutics and.
    3. 3Pharmacology, School of Pharmaceutical Education and Research, Jamia Hamdard, New Delhi, India.
    4. 4Department of Health Information Technology, Jeddah Community College, King Abdulaziz University, Jeddah, Kingdom of Saudi Arabia.

    CORRESPONDENCE

    Mohd. Mujeeb

    Pharmaceutics and.

    mohdmujeeb72@gmail.com

    Received: 02-08-2019; Revised: 31-10-2019.

    Volume 16, Issue 68S · pp. S39–S46 · PUBLISHED 31 March 2020 · DOI: 10.4103/pm.pm_345_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: Comparing traditional and modern extraction modes for extraction of lawsone from L. inermis leaves and simultaneous use of response surface methodology for optimizing its extraction procedure. Materials and Methods: Design‑Expert software was exploited for optimization task. Quantification of lawsone in different extracts was done by high‑performance liquid chromatography using aqueous acetic acid and methanol in the mobile phase. Results: Ultrasound‑assisted extraction stood out to be a supreme technique among all. Moreover, of all solvents (ethylene glycol, dimethylformamide, and methanol) examined methanol stood out to be the most effective solvent for lawsone extraction. By the inverse matrix of the regression model and point prediction, optimal conditions for lawsone extraction were laid down as ‑ extraction temperature ‑ 50.24°C, extraction time ‑ 15.70 min, liquor to material ratio ‑ 24.16 mL/g, and methanol concentration ‑ 75.15%v/v which yielded 17.129 g of lawsone. Under similar conditions (extraction temperature ‑ 50°C, extraction time ‑ 16 min, liquor to material ratio ‑ 24 mL/g, and methanol concentration ‑ 75%v/v), 16.98 g of lawsone was yielded which was close to predicted value. Conclusion: We conclude that a non-thermal method proved to a supreme technique for lawsone extraction which has an additional benefit which of avoiding thermal degradation of compound. Furthermore, by model fitting and analysis of regression coefficients, it was confirmed that all the factors studied significantly affected lawsone yield.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Abidin, L., Mujeeb, M., Aqil, M., Najmi, A. K., & Ahmad, A. (2020). Computer-Aided Box–Behnken Outlook Towards Optimization of Extraction of Lawsone from Mehendi Leaves. Pharmacognosy Magazine, 16(68S), S39–S46. https://doi.org/10.4103/pm.pm_345_19