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    Antiprotease Activity of Indigenous Medicinal Plants against Pakistani Echis carinatus Venom

    Nazia Aslam2, Tariq Javed3, Sofia Khalid2, Nusrat Shaheen4, Syeda Fatima2, Muhammad Latif5, Khurram Afzal6, Samina Afzal7, Khizar Abbas7, Muhammad Arfat Yameen8, Yasser M. S. A. Al-Kharaman8, Muhammad Imran Amirzada8, Ryan J. R. McCleary9, Albert A. Rizvanov10, Muhammad Hassham Hassan Bin Asad8,10★★ Corresponding author

    1. 1Department of Environmental Sciences, Fatima Jinnah Women University, Rawalpindi.
    2. 2Department of Pharmacy, LMDC, University of Health Sciences, Lahore.
    3. 3Department of Chemistry, Abbottabad University of Science and Technology, Abbottabad.
    4. 4Department of Zoology, Division of Science and Technology, University of Education, Multan Campus.
    5. 5Institute of Food Sciences, Bahauddin Zakariya University.
    6. 6Faculty of Pharmacy, Bahauddin Zakariya University, Multan.
    7. 7Department of Pharmacy, COMSATS University Islamabad, Abbottabad Campus, Pakistan.
    8. 8Department of Biology, 421 N, Woodland Blvd, Stetson University, DeLand, Florida, United States.
    9. 9Department of Genetics, Institute of Fundamental Medicine and Biology, Kazan Federal University, Kazan, Russia.

    CORRESPONDENCE

    Muhammad Hassham Hassan Bin Asad

    Department of Biology, 421 N, Woodland Blvd, Stetson University, DeLand, Florida, United States.

    hasshamasad@yahoo.com

    Received: 10-08-2019; Revised: 10-10-2019; Accepted: 09-01-2020.

    Volume 16, Issue 69 · pp. 416–421 · PUBLISHED 15 June 2020 · DOI: 10.4103/pm.pm_355_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This study was conducted to investigate the inhibitory potential of extracts from Pakistani medicinal plants against protease enzyme abundant in Echis carinatus venom. Materials and Methods: Organic extracts from different indigenous plant species and parts were used for in vitro determination of their inhibition against protease activity in snake venom. Active methanolic extracts were further fractioned using different solvents, and these fractions were also tested for antiprotease activity. Results: Results of this study show that Calotropis procera (Wild.) R. Br., Matthiola incana (L.) R. Br., and Terminalia arjuna Wight and Arn were able to neutralize the protease enzyme by 63%, 71%, and 66%, respectively. Trichodesma indicum (L.) R. Br. showed 51% inhibition of protease activity. Conclusion: The present study indicated about C. procera (Wild.) R. Br., M. incana (L.) R. Br., and T. arjuna Wight and Arn possessed inhibitor (s) against protease enzyme present in E. carinatus venom and would be worthwhile for development as treatment against envenomation in future.

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      Aslam, N., Javed, T., Khalid, S., Shaheen, N., Fatima, S., Latif, M., Afzal, K., Afzal, S., Abbas, K., Yameen, M. A., Al-Kharaman, Y. M. S. A., Amirzada, M. I., McCleary, R. J. R., Rizvanov, A. A., & Asad, M. H. H. B. (2020). Antiprotease Activity of Indigenous Medicinal Plants against Pakistani Echis carinatus Venom. Pharmacognosy Magazine, 16(69), 416–421. https://doi.org/10.4103/pm.pm_355_19