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    Article

    Potential Antileptospiral Constituents from Phyllanthus amarus

    Chandan S1★, Umesha S2, Shiva Prasad K3, Balamurugan V4, Chandrashekar S5, Santosh Kumar S R6, Ramith Ramu1, Prithvi S Shirahatti7, Asad Syed8, Abdallah M. Elgorban8★ Corresponding author

    1. 1Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research.
    2. 2Department of Studies in Biotechnology, University of Mysore, Manasagangotri.
    3. 3Department of Sciences, Amrita School of Arts and Sciences, Amrita Vishwa Vidyapeetham, Mysuru Campus, Mysuru.
    4. 4Indian Council of Agricultural Research-National Institute of Veterinary Epidemiology and Disease Informatics, Yelahanka, Bengaluru, Departments of.
    5. 5Biotechnology and.
    6. 6Food Technology, Davangere University, Shivagangothri, Davangere.
    7. 7Department of Biotechnology, Teresian College, Siddhartha Nagara, Mysuru, Karnataka, India.
    8. 8Department of Botany and Microbiology, College of Science, King Saud University, Riyadh 11451, Saudi Arabia.

    CORRESPONDENCE

    Chandan S

    Department of Biotechnology and Bioinformatics, School of Life Sciences, JSS Academy of Higher Education and Research.

    chandans@jssuni.edu.in

    Received: 10-01-2020; Revised: 20-02-2020; Accepted: 21-04-2020.

    Volume 16, Issue 70S · pp. S371–S378 · PUBLISHED 28 August 2020 · DOI: 10.4103/pm.pm_10_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The aim of the study is to investigate antileptospiral activity and isolate the potential antileptospiral constituents of the methanol extract of PA (MPA). Materials and Methods: The primary pharmacological tests for leptospirosis were investigated by test tube dilution technique and microdilution technique. To examine the morphogenesis of experimental leptospirosis by morphologic and histological methods, albino mice were inoculated intraperitoneally with Leptospira interrogans sero group Icterohaemorrhagiae strains. Results: The activity-guided repeated fractionation for MPA through silica gel column chromatography yielded three compounds that exhibited antioxidant and in vitro, in vivo, and in silico antileptospiral activities. Based on diverse physicochemical and spectroscopic analyses (viz., 13C NMR,1H NMR, ultraviolet [UV], IR, and mass spectroscopy), the potential constituents were elucidated as 5-(3-(3,4-dimethoxybenzyl)-4-methoxy-2-(methoxymethyl)butyl)- 4,7-dimethoxybenzo[d][1,3]dioxole(C1),1-(3-(3,4-dimethoxybenzyl)-4- methoxy-2-(methoxymethyl)butyl)-2,3,4,5tetramethoxybenzene(C2), and 4-(3-(3,4dimethoxybenzyl)-4-methoxy-2-(methoxymethyl)butyl)-3,6- dimethoxybenzene-1,2-diol (C3). The histopathological examinations of both kidney and liver showed promising activity with C3 at 75 and 100 µg/mL, respectively. Conclusion: The in vitro, in vivo, and in silico studies revealed that benzo methoxy class of compounds has great potential as antileptospiral agents.

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      S, C., S, U., K, S. P., V, B., S, C., R, S. K. S., Ramu, R., Shirahatti, P. S., Syed, A., & Elgorban, A. M. (2020). Potential Antileptospiral Constituents from Phyllanthus amarus. Pharmacognosy Magazine, 16(70S), S371–S378. https://doi.org/10.4103/pm.pm_10_20