Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    In vitro Antitumor Potential of Methanol Extract of Mimosa pudica in Human Breast Cancer Cell Lines

    Reni John1, Bibu John Kariyil2★, P. T. A. Usha1, S. Surya1, G. Anu1, Preethy John1, S. Sujith1, Aziz Zarina3★ Corresponding author

    1. 1Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, India.
    2. 2Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Wayanad, India.
    3. 3Department of Veterinary Physiology, College of Veterinary and Animal Sciences, Mannuthy, Thrissur, Kerala, India.

    CORRESPONDENCE

    Bibu John Kariyil

    Department of Veterinary Pharmacology and Toxicology, College of Veterinary and Animal Sciences, Pookode, Wayanad, India.

    bibujohn@kvasu.ac.in

    Received: 26-12-2019; Revised: 26-02-2020; Accepted: 21-04-2020.

    Volume 16, Issue 70S · pp. S396–S403 · PUBLISHED 28 August 2020 · DOI: 10.4103/pm.pm_527_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The present study was conducted to assess the anticancer activity of methanol extract of whole plant of M. pudica (MMP) in MCF‑7 and MDA‑MB‑231 breast cancer cell lines. Materials and Methods: The antitumor activity of MMP was analyzed using 3‑(4,5‑dimethyl thazol‑2‑yl)‑2, 5‑diphenyl tetrazolium bromide assay in MCF‑7 and MDA‑MB‑231 cell lines. The cytological and metabolic alterations due to MMP were evaluated using acridine orange/ethidium bromide dual staining, hoechst 33258 and fluoroprobe, benzimidazol‑carbocyanine iodide 5, 5’, 6, 6’‑tetrachloro‑1, 1’, 3,3’‑tetra ethyl (JC‑1) staining. Real‑time polymerase chain reaction and Western blotting were carried out to assess Bcl‑2, the antiapoptotic gene, and protein expression, respectively. Qualitative analysis and gas chromatography high‑resolution mass spectrometry were performed for the presence of various phytochemicals. Results: The methanol extract exhibited a potent antitumor activity in both the cell lines in vitro. Conclusion: The plant extract was showing the mode of action through intrinsic pathway of apoptotic cell death and may be studied further to develop a potent drug against breast cancer.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      John, R., Kariyil, B. J., Usha, P. T. A., Surya, S., Anu, G., John, P., Sujith, S., & Zarina, A. (2020). In vitro Antitumor Potential of Methanol Extract of Mimosa pudica in Human Breast Cancer Cell Lines. Pharmacognosy Magazine, 16(70S), S396–S403. https://doi.org/10.4103/pm.pm_527_19