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    Pharmacognosy MagazineNOW IN
    Article

    Evaluation of Antioxidant, Anti-inflammatory, and Analgesic Activities of Cissus vitiginea L. Leaves

    Beibei Chen1★, Jinfa Tang1★, Ming Niu4, Ruyuan Zhu2, Lin Li2, Lili Wang5, Yimiao Tian2, Rui Li2, Qiangqiang Jia2, Dandan Zhao2, Fangfang Mo2, Elena B. Romanenko6, Alexander N. Orekhov7, Sihua Gao2, Dieter Brömme8, Dongwei Zhang2★★ Corresponding author

    1. 1Diabetes Research Center, Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing.
    2. 2The First Affiliated Hospital of Henan University of Traditional Chinese Medicine, Zhengzhou.
    3. 3TCM Research Institute of General PLA, 302 Hospital.
    4. 4Modern Research Center of TCM, Chinese Material Medical School, Beijing University of Chinese Medicine, Beijing, China.
    5. 5Department of Molecular Basis of Ontogenesis, Belozersky Institute of Physical and Chemical Biology, Moscow State University.
    6. 6Laboratory of Angiopathology, Institute of General Pathology and Pathophysiology, Russian Academy of Medical Sciences, Moscow, Russia.
    7. 7Faculty of Dentistry, University of British Columbia, Vancouver, Canada.

    CORRESPONDENCE

    Beibei Chen

    Diabetes Research Center, Traditional Chinese Medicine School, Beijing University of Chinese Medicine, Beijing.

    dongwei1006@gmail.com

    Received: 29-06-2019; Revised: 01-10-2019; Accepted: 17-02-2020.

    Volume 16, Issue 71 · pp. 471–478 · PUBLISHED 20 October 2020 · DOI: 10.4103/pm.pm_278_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: The objective was to develop a sensitive, rapid, and accurate ultra‑performance liquid chromatography‑mass spectrometry (UPLC‑MS) method for the determination of the pharmacokinetics profile of salidroside after oral administration of FLL aqueous extract in normal and ovariectomized (OVX) rats. Materials and Methods: OVX and normal rats were orally administrated with FLL at a bolus of 7 g/kg. Plasma samples were precipitated by methanol, and the supernatant was chromatographed by a Waters BEH C18 column with a gradient elution of ammonium acetate and acetonitrile. Quantification was carried out on the electrospray ionization, positive multiple reaction monitoring modes. Results: The lower limit of detection was 50 ng/mL, and the dynamic linear range was 50–30,000 ng/mL with a value of R2 > 0.99. The intra‑ and inter‑day precisions were lower than 14.67%, and accuracy was in the range of 99.29%–103.37%. The recovery of salidroside ranged from 88.90% to 101.78%, with the matrix effect ranging from 85.53% to 100.45%. The t1/2, MRT0–∞, and apparent volume of distribution for salidroside increased in OVX rats. Conclusion: A sensitive, accurate, and rapid method was successfully established and validated for the determination of plasma characteristics of salidroside in Sprague–Dawley (SD) rats. The results suggest that ovariectomy could interfere with salidroside metabolism in SD rats.

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      Chen, B., Tang, J., Niu, M., Zhu, R., Li, L., Wang, L., Tian, Y., Li, R., Jia, Q., Zhao, D., Mo, F., Romanenko, E. B., Orekhov, A. N., Gao, S., Brömme, D., & Zhang, D. (2020). Evaluation of Antioxidant, Anti-inflammatory, and Analgesic Activities of Cissus vitiginea L. Leaves. Pharmacognosy Magazine, 16(71), 471–478. https://doi.org/10.4103/pm.pm_278_19