Volume 16, Issue 71

Original Article

1. Article

   Methanolic Extract of Mitragyna speciosa Korth Leaf Exhibits Place Preference Only at Higher Doses in Mice

   Kamini Vijeepallam, Vijayapandi Pandy, Dharmani Devi Murugan, Murali Naidu Kuppusamy

   pp. 449–454 · DOI: 10.4103/pm.pm_62_20 · Published 20 October 2020

   Objectives: The purpose of this research was to investigate the reinforcing properties of M. speciosa Korth leaf extract obtained via methanol extraction using mouse conditioned place preference (CPP) test. Materials and…

2. Article

   Apigenin attenuated ethylene glycol induced urolithiasis in uninephrectomized hypertensive rats: A possible role of bikunin, BMP‑2/4, and osteopontin

   Ke Zhang, Amit Kandhare, Anwesha Mukherjee‑Kandhare, Subhash L. Bodhankar

   pp. 455–463 · DOI: 10.4103/pm.pm_83_20 · Published 20 October 2020

   Aim: To determine the protective effect of apigenin in experimental ethylene glycol (EG)‑induced urolithiasis in uninephrectomized hypertensive animals. Materials and Methods: EG was administration in uninephrectomized r…

3. Article

   Neuroprotective Effect of the Essential Oil of Lavandula officinalis against Hydrogen Peroxide‑induced Toxicity in Mice

   Sarra Akermi, Jackson Roberto Guedes da Silva Almeida, Ahd Khedher, Hafsia Bouzenna, Sabah Dhibi, Anouar Feriania, Afoua Mufti, Amal Daoud, Abdelfattah Elfeki, Najla Hfaiedh

   pp. 464–470 · DOI: 10.4103/pm.pm_460_19 · Published 20 October 2020

   Background: Brain is the main organ that manages all other organs and has significant oxygen requirements, which makes it vulnerable to reactive oxygen species, thus causing different neurological disorders. The aim of t…

4. Article

   Evaluation of Antioxidant, Anti-inflammatory, and Analgesic Activities of Cissus vitiginea L. Leaves

   Beibei Chen, Jinfa Tang, Ming Niu, Ruyuan Zhu, Lin Li, Lili Wang, Yimiao Tian, Rui Li, Qiangqiang Jia, Dandan Zhao, Fangfang Mo, Elena B. Romanenko, Alexander N. Orekhov, Sihua Gao, Dieter Brömme, Dongwei Zhang

   pp. 471–478 · DOI: 10.4103/pm.pm_278_19 · Published 20 October 2020

   Objective: The objective was to develop a sensitive, rapid, and accurate ultra‑performance liquid chromatography‑mass spectrometry (UPLC‑MS) method for the determination of the pharmacokinetics profile of salidroside aft…

5. Article

   Narirutin Suppresses M1‑Related Chemokine InterferonGamma-Inducible Protein‑10 Production in Monocyte‑Derived M1 Cells via Epigenetic Regulation

   Chung‑Hsiang Li, Lin Wang, Chang‑Hung Kuo, Yi‑Ting Chen, Mei‑Lan Tsai, Chih‑Hsing Hung

   pp. 479–485 · DOI: 10.4103/pm.pm_105_20 · Published 20 October 2020

   Objective: We try to investigate this anti‑inflammatory effect of narirutin in human monocytic THP‑1‑derived M1 macrophage cells. Materials and Methods: To confirm our hypothesis, human THP‑1 cells (1 × 106 cells/mL) wer…

6. Article

   Effect of Diosgenin on T‑Helper 17 Cells in Mice with Collagen‑Induced Arthritis

   Yaxian Gao, Yongwei Wang, Hongru Song, Yachun Guo, Enhong Xing, Xiaofei Zhao, Wei Li, Junxia Zhang, Chunyan Yu

   pp. 486–492 · DOI: 10.4103/pm.pm_426_19 · Published 26 August 2020

   Objective: The objective of this study is to explore the therapeutic effects of diosgenin against collagen‑induced arthritis (CIA) in DBA/1J mice. Materials and Methods: The CIA model was established using chicken type I…

7. Article

   Immunostimulatory Activities of a High Molecular Weight Fraction of Cynanchum auriculatum Royle ex Wight Root Obtained by Ultrafiltration

   Mi Jang, Tae‑Gyu Lim, Eunjung Lee, Hee‑Do Hong, Young Kyoung Rhee, Yun Ji Lee, Jeong Hoon Lee, Chang‑Won Cho

   pp. 493–498 · DOI: 10.4103/pm.pm_449_19 · Published 20 October 2020

   Objectives: In this study, we explored the immune‑enhancing effect of the high‑molecular weight fraction of C. auriculatum (CAHF) root extracts in RAW 264.7 macrophages and cyclophosphamide (CYP)‑induced immune‑suppresse…

8. Article

   Anticancer Activity of Juniperus procera Grown in Southwestern Region of Saudi Arabia on Human Oral Squamous Cell Carcinoma Cell Lines

   Abdulaziz AlShahrani, Ibrahim AlShahrani, Jagadish Hosmani, Rafi Ahmed Togoo, Tasneem Sakinatulain, Tanveer Alam, Mohammad Shahul Hameed

   pp. 499–509 · DOI: 10.4103/pm.pm_28_20 · Published 20 October 2020

   Objectives: The objective was to evaluate the anticancer properties of JP from Al‑Baha region on SCC‑9 cells. Materials and Methods: Colorimetric assays such as 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyl tetrazolium bromi…

9. Article

   Protective Effects of Gracilaria lemaneiformis Extract against Ultraviolet B‑Induced Damage in HaCaT Cells

   Yingnian Lu, Si Mei, Pan Wang, Peipei Ouyang, Xuehua Liao, Hua Ye, Kefeng Wu, Xiaoli Ma

   pp. 510–517 · DOI: 10.4103/pm.pm_437_19 · Published 20 October 2020

   Background: Gracilaria lemaneiformis is an edible red marine macroalga that contains various active components including phycoerythrin, polysaccharides, and phenolics. In our previous work, crude ethanolic extracts of G.…

10. Article

    Involvement of Toll‑like Receptor 2/Myeloid Differentiation Factor 88/Nuclear Factor kappa B/NLR Family Pyrin Domain‑containing 3 Signaling Pathways in the Hepatoprotective Effect of Lagotis brachystachys in Rats with Alcoholic Liver Disease

    Ji Xiao Zhu, Jia Ling Shan, Xiang Ouyang, Hai Yan Yang, Rong Rui Wei, Jin Xiang Zeng, Hong Yu Cheng

    pp. 518–523 · DOI: 10.4103/pm.pm_557_19 · Published 20 October 2020

    Objectives: The objective of the study was to evaluate the hepatoprotective effect of whole extracts of L. brachystachys against chronic alcohol‑induced ALD in rats. Materials and Methods: Different polar compounds of et…

11. Article

    Honey as a Solvent for the Green Extraction, Analysis, and Bioconversion of Daidzin from Pueraria candollei var. mirifica Root

    Suppalak Phaisan, Gorawit Yusakul, Poomraphie Nuntawong, Seiichi Sakamoto, Waraporn Putalun, Satoshi Morimoto, Hiroyuki Tanaka

    pp. 524–530 · DOI: 10.4103/pm.pm_74_20 · Published 20 October 2020

    Objectives: This study aims to apply honey (H‑NADES) and a sugar‑based NADES (S‑NADES) for the extraction, analysis, and bioconversion of daidzin from Pueraria candollei var. mirifica (PM) root. Materials and Methods: Va…

12. Article

    Chlorogenic Acid in Viscum album Callus is a Potential Anticancer Agent against C6 Glioma Cells

    Jinwoo Kim, Suji Baek, Kang Pa Lee, Byung Seok Moon, Hyun-Soo Kim, Seung-Hae Kwon, Dae won Lee, Jisu Kim

    pp. 531–537 · DOI: 10.4103/pm.pm_479_19 · Published 20 October 2020

    Objective: Our study aimed to explore the anticancer effects of CA obtained from Viscum album callus on C6 glioma cell migration and proliferation. Materials and Methods: Anticancer potency was analyzed by the 2,3‑bis‑(2…

13. Article

    Identification of Polygonatum odoratum Based on Support Vector Machine

    Zhong Li, Jie Zheng, Qin Long, Yi Li, Huaying Zhou, Tasi Liu, Bin Han

    pp. 538–542 · DOI: 10.4103/pm.pm_410_19 · Published 20 October 2020

    Objectives: We aimed to establish a reliable and accurate classification model of P. odoratum based on the support vector machine (SVM) and identify it from different habitats; we also aimed to identify its adulterants. …

14. Article

    Preparation, Characteristics, and Antioxidant Activity of the Selenium Nanoparticles Stabilized by Polysaccharides Isolated from Grateloupia filicina

    Bilang Cao, Yueling Yang, Chunhua Yue, Yuanyuan Wang, Pengcheng Fu, Yongguang Bi

    pp. 543–549 · DOI: 10.4103/pm.pm_405_19 · Published 20 October 2020

    Objectives: In this study, we aimed to optimize the extraction parameters of polysaccharides of Grateloupia filicina (GFPs) and investigate the antioxidant activity of SeNPs stabilized by GFPs. Materials and Methods: GFP…

15. Article

    Anticancer Effects of Calotropis procera Latex Extract in MCF‑7 Breast Cancer Cells

    Mohammed A. M. Al‑Qahtani, Mohammad Abul Farah, Faisal M. Abou‑Tarboush, Khalid M. Al‑Anazi, Naif O. Al‑Harbi, Mohammad Ajmal Ali, Waleed A. Q. Hailan

    pp. 550–556 · DOI: 10.4103/pm.pm_156_20 · Published 20 October 2020

    Objectives: The present study was conducted to isolate a cardiac glycosidal (CG) extract from the latex of C. procera and to assess its potential in inducing anticancer effects on breast cancer cells (MCF‑7). Materials a…

16. Article

    Isovitexin, A New Metabolite, Was Found in the Metabolites of Co-cultured Five Flavonoids Isolated from Ziziphus jujuba Mill var. spinosa Seeds by Rat Intestinal Flora

    Ying Liu, Junbo Xie, Xusheng Cui, Yanqing Zhang, Kunsheng Zhang

    pp. 557–563 · DOI: 10.4103/pm.pm_454_19 · Published 20 October 2020

    Objectives: The primary objective of this study was to investigate the mechanism of degradation of co‑cultured flavonoids, namely 6´´´‑feruloyl spinosin, 6´´´‑p‑coumaroyl spinosin, spinosin, swertisin, and isovitexin, in…

17. Article

    Chemical Constituents from Saussurea pachyneura

    Shuo Zhang, Xiao‑Man Tu, Wan‑Chang Zhang, Guang‑Bo Xie

    pp. 564–567 · DOI: 10.4103/pm.pm_453_19 · Published 20 October 2020

    Objectives: Saussurea pachyneura, a plant from genus Saussurea, was studied for its secondary metabolites. Materials and Methods: The air‑dried whole plant material was extracted by ethanol. The compounds from the ethano…

18. Article

    Synthesis, Characterization, and Biological Activity of Silver Nanoparticles Synthesized from Aristolochia bracteolata Lam.

    Nagaraja Sreeharsha, Govindarajan Kathiravan, Kalyanaraman Rajagopal, Arvindganth Rajasekar, Bose Rajangam, Bandar E. Aldhubiab, Venugopala Narayanaswamy, Mahesh Attimarad, Anroop B. Nair, Rajith Kumar Karnathi, Girish Meravanige, Uma Chandur, Teeka S. Roopashree, Micheline Haroun, Christophe Tratrat

    pp. 568–573 · DOI: 10.4103/pm.pm_313_20 · Published 20 October 2020

    The aim of this research is to analyze the antimicrobial activity and cytotoxicity of biologically synthesized silver nanoparticles against A549 cancer cell line. Silver nanoparticles were synthesized from the leaf extra…

19. Article

    Isoliquiritigenin Induces Apoptosis through Caspases and Reactive Oxygen Species Signaling Pathways in Human Bladder Cancer Cells

    Minwoo Hwang, Min Ji Kwon, Byung Joo Kim

    pp. 574–579 · DOI: 10.4103/pm.pm_21_20 · Published 20 October 2020

    Objectives: Mechanisms underlying ISL‑induced cell death were investigated in 5637 human bladder cancer cell line. Materials and Methods: Cell viabilities were measured with 3‑[4,5‑dimethylthiazol‑2‑yl]‑2,5‑diphenyltetra…

20. Article

    Analgesic, Anti‑Inflammatory, and Central Nervous System Depressant Activities of Monochoria hastate (L.) Solms. in Animal Models

    Tufael Ahmed, Luthfun Mst. Nesa, D. A. Anwar Al‑Aman, Islam Nazrul Kazi, Md. Eleas Kobir, Md. Ahsan Ullah, Dabanshu Dhali, Mohammad Abdur Rashid

    pp. 580–584 · DOI: 10.4103/pm.pm_463_19 · Published 20 October 2020

    Objectives: The objective of the study was to investigate the pharmacological effect of crude leaf extracts of M. hastate in mice model, justifying its medicinal use and evaluating its safety and efficacy as a crude drug…

21. Article

    Protective Effect of Caffeic Acid Phenethyl Ester against Acute and Subchronic Mice Cardiotoxicity Induced by Cyclophosphamide Alone or Plus Naproxen

    Promise Madu Emeka, Mohamed A. Morsy, Ibrahim A. Alhaider, Muhammad S. Chohan

    pp. 585–591 · DOI: 10.4103/pm.pm_159_20 · Published 20 October 2020

    Objectives: This study aims to investigate the possible protective effect of caffeic acid phenethyl ester (CAPE) in the co‑administration of CYP and naproxen (NAP) with acute and subchronic treatments in mice. Materials …

22. Article

    Profiling of Antiviral and Antioxidant Phytochemicals of Pterocephalus frutescens Hochist. using High‑Resolution Ultra‑Performance Liquid Chromatography/Quadrupole Time‑of‑Flight Mass Spectrometer

    Atef A. El-Hela, Mostafa M. Hegazy, Marwa S. Abu Bakr, Hatem S. Abbass

    pp. 592–599 · DOI: 10.4103/pm.pm_558_19 · Published 20 October 2020

    Background: Medicinal plant extracts and herbal preparations are complex mixtures of active and ballast substances, which may contain in frequently up to several hundreds of different constituents, with no exactly define…

23. Article

    Polygonum chinense Water Decoction Lessens Acute Lung Injury in Mice Induced by Influenza Virus

    Rongkui Liu, Zhao Zhang, Xueyang Peng, Yueling Su, Yan Xiao, Xiuli Ji

    pp. 600–604 · DOI: 10.4103/pm.pm_206_19 · Published 20 October 2020

    Background: In traditional Chinese medicine, Polygonum chinense has been used to treat influenza. However, little information is available from current studies regarding the anti‑influenza pharmacological activities of P…

24. Article

    Fisetin Attenuates Gastric Mucosal Lesions through Modulating Nuclear Factor‑Kappa B and Peroxisome Proliferator‑Activated Receptor‑γ in Rats

    Jinchen Hu, Li Cai, Zengwu Yao, Zhenbin Zhang, Menglai Zhang, Yifei Zhang, Lixin Jiang, Baohong Hu

    pp. 605–612 · DOI: 10.4103/pm.pm_4_20 · Published 20 October 2020

    Objective: The objective of this study was to explore the underlying mechanism of action of fisetin on ethanol‑induced gastric ulcer model. Materials and Methods: In this study, gastric mucosal lesions were induced by et…

25. Article

    Rehmannia radix Extract Ameliorates Imiquimod‑Induced Psoriasis‑Like Skin Inflammation in a Mouse Model via the Janus‑Kinase Signal Transducer and Activator of Transcription Pathway

    Haixiu Zhang, Minyan Dang, Xuemei Chen, Xin Yan

    pp. 613–619 · DOI: 10.4103/pm.pm_218_19 · Published 20 October 2020

    Background: Rehmannia glutinosa, commonly known as Dihuang, is a medicinal herb widely used in the formulations of traditional Chinese medicine. Radix or the root of Rehmannia is a vital ingredient used in various orient…

26. Article

    Naringenin Reduced Migration in Osteosarcoma Cells through Downregulation of Matrix Metalloproteinase‑2 and Matrix Metalloproteinase‑9 and Snail

    Yuh‑Ming Chang, Ming‑Cheng Lin, Ling‑Zong Hong, Pei‑Ni Chen, Yu‑Hsin Tsai, You‑Cheng Hseu, Ke‑Min Chen, Li‑Sung Hsu, Ting‑Hsien Kao

    pp. 620–624 · DOI: 10.4103/pm.pm_31_20 · Published 20 October 2020

    Objectives: In this study, we investigate the effects of naringenin on osteosarcoma. Materials and Methods: 3‑(4,5‑dimethylthiazol‑2‑yl)‑2,5‑diphenyltetra zolium bromide (MTT) assay was performed to detect the cell viabi…

27. Article

    A Special Aromatic Chrysanthemum Breed with High Content of Thujone

    Ying Zhang, Risheng Cheng, Zijie Xu, Yaoyao She, Keli Chen, Yimei Liu

    pp. 625–629 · DOI: 10.4103/pm.pm_382_19 · Published 20 October 2020

    Objective: The objective of the study was to determine taxonomic status of the cultivar in genus Dendranthema. Materials and Methods: The differences of morphological characteristics and volatile oil components between t…

28. Article

    Effect and Mechanism of Ginsenoside Rg1 on Synaptic Plasticity of Oxygen‑Glucose Deprivation/Reoxygenation‑Induced Neuronal Injury

    Chen Yan, Yan Ma, Lin Li, Meng Zhang, Junhua Zhan, Xiang Fan

    pp. 630–636 · DOI: 10.4103/pm.pm_541_19 · Published 20 October 2020

    Objective: In this study, we aim to establish the model of oxygen–glucose deprivation/reoxygenation (OGD/ R)‑injured primary cortical neurons to mimic ischemia/reperfusion injury and investigate the mechanisms of action …

29. Article

    Sagittaria sagittifolia Polysaccharide, a Chinese Herbal Extract, Protects against Isoniazid‑ and Rifampicin‑induced Hepatotoxicity in in-vitro Model

    Yibo Tang, Junping Lv, Jing Wang, Bing Li, Qiaohui Zhang, Zhenquan Liu, Xiuhui Ke, Weizao Luo, Yin Lin, Yan Liao

    pp. 637–643 · DOI: 10.4103/pm.pm_542_19 · Published 20 October 2020

    Objectives: In this study, we aimed to investigate the protective effect and the underlying mechanisms of SSP in cultured HepG2 cells after induction with INH and RFP. Materials and Methods: The study was designed as fol…

30. Article

    Methanolic Extracts of Capparis ecuadorica Iltis Inhibit the Inflammatory Response in Lipopolysaccharide‑Stimulated RAW 264.7 Macrophage Cells

    Bo Ram Song, Ji Eun Kim, Jin Ju Park, Mi Rim Lee, Jun Young Choi, Jin Kyung Noh, Hyun Keun Song, Dae Youn Hwang

    pp. 644–653 · DOI: 10.4103/pm.pm_464_19 · Published 20 October 2020

    Objectives: In this study, we investigated the anti‑inflammatory activity of a methanolic extract of C. ecuadorica leaves (MCE) in macrophages. Materials and Methods: Anti‑inflammatory responses and mechanisms were asses…

31. Article

    Distinguishing the Rhizomes of Atractylodes japonica, Atractylodes chinensis, and Atractylodes lancea by Comprehensive Two‑Dimensional Gas Chromatography Coupled with Mass Spectrometry Combined with Multivariate Data Analysis

    Jie Lu, WenTing Chen, BoWen Zhou, Yang Chen, XinHong Wang, Rui An, Ming Yang

    pp. 654–661 · DOI: 10.4103/pm.pm_33_20 · Published 20 October 2020

    Background: In clinical practice, the species of Atractylodes are difficult to identify based on their morphological and chemical features which often leads to confusion. In addition, the composition of volatile componen…

32. Article

    Content Determination and Anti-fatigue Effect of the Purified Anthocyanin from Purple Daucus carota

    Fang-Rong Cheng, Yue-Yue Mao, Song-Heng Jin, Rong-Rong Cai, Ke Yuan

    pp. 670–674 · DOI: 10.4103/pm.pm_452_19 · Published 20 October 2020

    Background: The genus <em>Cissus</em> (Vitaceae) is known for its medicinal properties. <em>Cissus quadrangularis</em> is a well-known medicinal plant used in traditional medicine for bone healing. Objective: The present…

33. Article

    Glabridin Downregulates Lipopolysaccharide‑Induced Oxidative Stress and Neuroinflammation in BV‑2 Microglial Cells via Suppression of Nuclear Factor‑κB Signaling Pathway

    Yan Wu, Jia Geng, Xiaoguang Lei, Qian Wu, Tao Chen, Lianmei Zhong

    pp. 675–680 · DOI: 10.4103/pm.pm_497_19 · Published 20 October 2020

    Background: Microglia initially undergoes chronic activation in response to the damages caused by stressful stimuli, such as bacterial infection and hypoxia. Inflammatory responses, as well as oxidative stress, perform c…

34. Article

    Fucoxanthin Modulates the Development of 7, 12-dimethyl benz (a) anthracene-induced Skin Carcinogenesis in Swiss Albino Mice in vivo

    Pengtao Kou, Najat Marraiki, Abdallah M. Elgorban, Yaowu Du

    pp. 681–688 · DOI: 10.4103/pm.pm_292_19 · Published 20 October 2020

    Background: Fucoxanthin (Fx), an distinctive carotenoid occurs on brown seaweed, contains several benefits including anti‑cancer effects. To investigate the chemopreventive effectual of Fx on 7,12‑dimethylbenz[a] anthrac…