Methanolic Extract of Mitragyna speciosa Korth Leaf Exhibits Place Preference Only at Higher Doses in Mice
Kamini Vijeepallam2, Vijayapandi Pandy2,3★, Dharmani Devi Murugan2, Murali Naidu Kuppusamy4★ Corresponding author
- 1Department of Pharmacology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
- 2Department of Pharmacology, Chalapathi Institute of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India.
- 3Department of Anatomy, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
CORRESPONDENCE
Vijayapandi Pandy
Department of Pharmacology, Chalapathi Institute of Pharmaceutical Sciences, Guntur, Andhra Pradesh, India.
Received: 01-03-2020; Revised: 07-04-2020; Accepted: 03-06-2020.
Volume 16, Issue 71 · pp. 449–454 · PUBLISHED 20 October 2020 · DOI: 10.4103/pm.pm_62_20
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ABSTRACT
Objectives: The purpose of this research was to investigate the reinforcing properties of M. speciosa Korth leaf extract obtained via methanol extraction using mouse conditioned place preference (CPP) test. Materials and Methods: In CPP study, following baseline preference test (preconditioning score), the mice were subjected to conditioning trials at varying doses of methanolic extract of M. speciosa (MMS, 50, 75, 100, 250, 500, and 1000 mg/kg, p.o.) or reference drugs methamphetamine (0.5 mg/kg, intraperitoneally [i.p.]) and clozapine (1 mg/kg, p.o.) or vehicle controls (1% w/v sodium carboxy methyl cellulose [10 mL/kg, p.o.] and saline [10 mL/kg, i.p.]) followed by a preference test performed under drug‑free state (postconditioning score). In addition, the effect of all tested drugs on the spontaneous locomotor activity was assessed. Results: The CPP study results revealed that MMS per se produced a significant place preference only at higher doses (>500 mg/kg, p.o.). Nevertheless, MMS at lower doses (50–250 mg/kg, p.o.) did not induce CPP in mice. In addition, MMS at all tested doses (50–1000 mg/kg, p.o.) did not affect the spontaneous locomotor activity in mice. Conclusion: MMS per se exhibits reinforcing properties at only an increased dose of >500 mg/kg, and therefore, it is best to administer at lower doses (<250 mg/kg) for the potential therapeutic benefits in preclinical studies.
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Vijeepallam, K., Pandy, V., Murugan, D. D., & Kuppusamy, M. N. (2020). Methanolic Extract of Mitragyna speciosa Korth Leaf Exhibits Place Preference Only at Higher Doses in Mice. Pharmacognosy Magazine, 16(71), 449–454. https://doi.org/10.4103/pm.pm_62_20