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    Factorial Design-Guided Optimization of Extraction of Therapeutically Active Furanocoumarin Khellin from Ammi majus L. Fruits

    Hina Kausar1, Lubna Abidin1, Mohd. Mujeeb1★, Mohd. Aqil2, Ozair Alam3★ Corresponding author

    1. 1Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education & Research (SPER), Jamia Hamdard, New Delhi, India.
    2. 2Pharmaceutics and, India.
    3. 3Pharmaceutical Chemistry, School of Pharmaceutical Education & Research (SPER), Jamia Hamdard, New Delhi, India.

    CORRESPONDENCE

    Mohd. Mujeeb

    Department of Pharmacognosy and Phytochemistry, School of Pharmaceutical Education & Research (SPER), Jamia Hamdard, New Delhi, India.

    mohdmujeeb72@gmail.com

    Received: 15-07-2020; Revised: 25-08-2020; Accepted: 24-09-2020.

    Volume 16, Issue 72 · pp. 830–835 · PUBLISHED 16 February 2021 · DOI: 10.4103/pm.pm_295_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The study objective was to analyze the optimization of extraction parameters of khellin from A. majus L. by response surface methodology (RSM). Materials and Methods: Box–Behnken Design (BBD) of RSM was used for optimization needs. Quantification of khellin in extracts was done by high performance thin-layer chromatography using a mix of ethyl acetate: toluene: formic acid, and the peaks were monitored at 254 nm. Results: Among the explored traditional modes and ultrasound‑assisted extraction (UAE), UAE was found to be the most ideal for khellin extraction, with methanol being the most suitable solvent. The applied BBD established a quadratic model for the experimental setup with the regression coefficient of 0.998. The optimal conditions were set up as – extraction temperature: 63.84°C, extraction time: 29.51 min. and solvent‑to‑drug ratio: 21.64 v/w, which yielded 6.21% w/w of khellin. Contrarily, under the modified experimental conditions, 6.86% w/w of khellin was extracted. Conclusion: It was concluded that all the variables studied significantly affected khellin yield. Moreover, a contemporary green extraction mode stood out to be the best for khellin extraction.

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      Kausar, H., Abidin, L., Mujeeb, M., Aqil, M., & Alam, O. (2021). Factorial Design-Guided Optimization of Extraction of Therapeutically Active Furanocoumarin Khellin from Ammi majus L. Fruits. Pharmacognosy Magazine, 16(72), 830–835. https://doi.org/10.4103/pm.pm_295_20