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    Inhibitory Effects of Triterpenoid Betulin on Inflammatory Mediators Inducible Nitric Oxide Synthase, Cyclooxygenase‑2, Tumor Necrosis Factor‑Alpha, Interleukin‑6, and Proliferating Cell Nuclear Antigen in 1,2‐Dimethylhydrazine‐Induced Rat Colon Carcinogenesis

    Jinfeng Yu2★, Min Li3★, Dong Zhan4, Chang Shi5, Le Fang6, Chunmei Ban7, Weifeng Zheng8, Vishnu Priya Veeraraghavan9, Surapaneni Krishna Mohan10, Xiaoling Tang11★★ Corresponding author

    1. 1Department General Medicine, Yantaishan Hospital, Yantai, Shandong.
    2. 2Medical College of Wuhan University, Wuhan, Hubei.
    3. 3Human Anatomy Laboratory of Experimental Teaching Centre, School of Basic Medical Sciences, Kunming Medical University, Kunming, Yunnan.
    4. 4Department of Traditional Chinese Medicine, Xinzhou District People’s Hospital, Wuhan, Hubei.
    5. 5Department of Clinical Laboratory, 521 Hospital of Ordnance Industry, Xi’an, Shaanxi, 710065.
    6. 6Department Oncology Liuzhou People’s Hospital, Liuzhou, Guangxi , 545006.
    7. 7Department of General Surgery, The 73th Group Army Hospital of the People’s Liberation Army, Success Hospital Affiliated To Xiamen University,Xiamen, Fujian,361003, China.
    8. 8Department of Biochemistry, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences, Saveetha University.
    9. 9Department of Biochemistry, Panimalar Medical College Hospital & Research Institute, (Affiliated to The Tamil Nadu Dr. MGR Medical University), Varadharajapuram, Poonamallee, Chennai, Tamil Nadu, India.
    10. 10Malignant Tumor TCM “Yi Qi Qing Du” Key Research Office, Jiangxi Academy of Traditional Chinese Medicine, Nanchang, Jiangxi, 330046, China.

    CORRESPONDENCE

    Jinfeng Yu

    Medical College of Wuhan University, Wuhan, Hubei.

    txl18942219160@sina.com

    Received: 29-11-2019; Revised: 28-01-2020; Accepted: 08-11-2020.

    Volume 16, Issue 72 · pp. 836–842 · PUBLISHED 16 February 2021 · DOI: 10.4103/pm.pm_516_19

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: In this study, we explored the anticancer, antiproliferative, and anti‑inflammatory effects of BE on 1,2‑dimethylhydrazine (DMH)‑treated rat model of colon cancer. Materials and Methods: Colon cancer was induced by a subcutaneous injection of DMH (20 mg/kg bwt) once a week for the initial 4 weeks of the experiment. We analyzed body weight, tumor incidence, tumor volume, total number of tumors, thiobarbituric acid reactive substances (TBARS), and levels of antioxidants (glutathione peroxidase, glutathione, catalase, and superoxide dismutase), bacterial enzymes (β‑glucuronidase and mucinase), Phase I (cytochrome P450 and cytochrome b5) and Phase II (GST and Glutathione reductase (GR)) detoxification enzymes, and inflammatory (cyclooxygenase‑2, interleukin‑1 beta [IL‑1β], inducible nitric oxide synthase, tumor necrosis factor‑alpha, and IL‑6) and cell proliferative (cyclin D1 and proliferating cell nuclear antigen) markers. We also assessed the histopathological alterations found in experimental and control rats. Results: We observed decreased body weight and levels of antioxidants and Phase II enzymes; augmented tumor incidence, tumor volume, total number of tumors, Phase I enzymes, TBARS, and levels of bacterial enzymes; and irregular histopathological changes in DMH‑treated rats. Moreover, the Western blotting analysis of colon tissues revealed upregulation of inflammatory and cell proliferative markers in DMH‑treated rats. Oral supplementation of 20 mg/kg bwt BE led to inhibition of tumor formation and inflammation, regulation of cell proliferation, and restoration of biochemical parameters. Our findings were supported by histopathological analysis. Conclusion: Our results suggested that BE exhibited anticancer, anti‑inflammatory, and antiproliferative effects against DMH‑induced colon cancer in rats.

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      Yu, J., Li, M., Zhan, D., Shi, C., Fang, L., Ban, C., Zheng, W., Veeraraghavan, V. P., Mohan, S. K., & Tang, X. (2021). Inhibitory Effects of Triterpenoid Betulin on Inflammatory Mediators Inducible Nitric Oxide Synthase, Cyclooxygenase‑2, Tumor Necrosis Factor‑Alpha, Interleukin‑6, and Proliferating Cell Nuclear Antigen in 1,2‐Dimethylhydrazine‐Induced Rat Colon Carcinogenesis. Pharmacognosy Magazine, 16(72), 836–842. https://doi.org/10.4103/pm.pm_516_19