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    Article

    Moringa oleifera Leaf Extract Exerts Antiproliferative Effects and Induces Mitochondria Mediated Apoptosis within Rat Glioblastoma (C6) Cells

    Ahmed Alafnan1, Talib Hussain1★, Abdulwahab Alamri1, Farhan Alshammari2, Afrasim Moin2, Syed Mohd Danish Rizvi2, K. L. Krishna3★ Corresponding author

    1. 1Department of Pharmacology and Toxicology, University of Hail, Hail, Saudi Arabia.
    2. 2Department of Pharmaceutics, University of Hail, Hail, Saudi Arabia.
    3. 3Department of Pharmacology, JSS College of Pharmacy, JSS Academy of Higher Education and Research, Mysore, Karnataka, India.

    CORRESPONDENCE

    Talib Hussain

    Department of Pharmacology and Toxicology, University of Hail, Hail, Saudi Arabia.

    mdth_ah@yahoo.com

    Received: 04-04-2021; Revised: 25-04-2021; Accepted: 03-05-2021.

    Volume 17, Issue 6S · pp. S240–S245 · PUBLISHED 16 July 2021 · DOI: 10.4103/pm.pm_145_21

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objective: The present study aims to evaluate the antiproliferative efficacy of ethanolic Mo leaf extract (MoEt‑OH) in mouse‑derived glioblastoma C6 cells. Materials and Methods: MoEt‑OH was prepared, and C6 cells were subjected to MoEt‑OH treatment at a dosage of 100, 200, and 400 µg/ml and incubated for 24 h. Results: Post-incubation, C6 cells exhibited a significant (P < 0.05) decline in their viability at 100 µg/ ml, which further increased proportionally with increase in MoEt‑OH concentration (P < 0.01; P < 0.001). MoEt‑OH significantly enhanced the lipid peroxidation as assessed by measuring the increased levels of malondialdehyde at 100 µg/ml (P < 0.05), 200 µg/ml (P < 0.01), and 400 µg/ ml (P < 0.001). MoEt‑OH‑mediated evaluation of glutathione levels also exhibited similar trends. Moreover, reactive oxygen species estimation revealed a substantial increase in oxidative stress posttreatment with MoEt‑OH within C6 cells, even in a dose‑dependent manner. MoEt‑OH also instigated apoptosis with glioblastoma cells through enhanced nuclear condensation and fragmentation as qualitatively evaluated through Hoechst 33342 staining. The apoptosis within C6 cells post‑MoEt‑OH treatment was linked with enhanced expressional levels of caspase‑9 and caspase‑3 proportional to the MoEt‑OH concentration. Conclusion: Thus, our preliminary study elucidated that MoEt‑OH treatment results in antiproliferation within C6 cells by enhancing oxidative stress and instigating apoptosis by initiating nuclear fragmentation.

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      Alafnan, A., Hussain, T., Alamri, A., Alshammari, F., Moin, A., Rizvi, S. M. D., & Krishna, K. L. (2021). Moringa oleifera Leaf Extract Exerts Antiproliferative Effects and Induces Mitochondria Mediated Apoptosis within Rat Glioblastoma (C6) Cells. Pharmacognosy Magazine, 17(6S), S240–S245. https://doi.org/10.4103/pm.pm_145_21