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    Article

    Carthami flos Induces Apoptosis by Activating Caspases and Regulating Mitogen‑Activated Protein Kinase and Reactive Oxygen Species Signaling Pathways in AGS Human Gastric Cancer Cells

    Song Ee Han1, Min Ji Kwon1, Jeong Nam Kim1, Eun Yeong Lim2,3, Yun Tai Kim2,3, Byung Joo Kim1★★ Corresponding author

    1. 1Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam-do.
    2. 2Division of Food Functionality, Korea Food Research Institute, Wanju.
    3. 3Department of Food Biotechnology, Korea University of Science and Technology, Daejeon, Republic of Korea.

    CORRESPONDENCE

    Byung Joo Kim

    Division of Longevity and Biofunctional Medicine, Pusan National University School of Korean Medicine, Yangsan, Gyeongsangnam-do.

    vision@pusan.ac.kr

    Received: 14-04-2020; Revised: 29-05-2020; Accepted: 13-08-2020.

    Volume 17, Issue 74 · pp. 307–314 · PUBLISHED 12 July 2021 · DOI: 10.4103/pm.pm_127_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The mechanisms of cell death induction by CF were investigated in AGS human gastric adenocarcinoma cells. Materials and Methods: Cell viability assay, cell cycle analysis, caspase activity assay, western blotting, and reactive oxygen species (ROS) assay were used to check the anti‑cancer effects of CF on AGS cells. Results: Treatment with CF (100500 μg/mL) inhibited AGS cell proliferation and increased the ratio of the subG1 phase of the cell cycle. CF induced cell death was associated with a reduction in Bcl-2 and an increase in Bax levels. Moreover, expression of the apoptosismediating surface antigen (FAS) was increased. CF activated caspase-3 and ‑9. In addition, it regulated the activation of mitogen‑activated protein kinases and increased intracellular ROS generation. Conclusion: These findings suggest that CF induced apoptosis in AGS cells and could thus, serve as a novel anticancer agent to promote apoptosis of gastric cancer cells.

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      Han, S. E., Kwon, M. J., Kim, J. N., Lim, E. Y., Kim, Y. T., & Kim, B. J. (2021). Carthami flos Induces Apoptosis by Activating Caspases and Regulating Mitogen‑Activated Protein Kinase and Reactive Oxygen Species Signaling Pathways in AGS Human Gastric Cancer Cells. Pharmacognosy Magazine, 17(74), 307–314. https://doi.org/10.4103/pm.pm_127_20