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    Article

    Lappaconitine Hydrochloride Induces Apoptosis and S Phase Cell Cycle Arrest through MAPK Signaling Pathway in Human Liver Cancer HepG2 Cells

    Na Song1, Junyi Ma1★, Xuemei Zhang1, Danni Qu1, Ling Hui2★, Chunyan Sang3, Haining Li4★ Corresponding author

    1. 1College of Life Science, Northwest Normal University, China.
    2. 2Key Laboratory of Stem Cells and Gene Drug of Gansu Provincial, The 940th Hospital of Joint Logistics Support Force of Chinese People’s Liberation Army, China.
    3. 3School of Pharmacy, Lanzhou University, China.
    4. 4Gansu Provincial Academy of Medical Sciences, Gansu Provincial Cancer Hospital, Lanzhou, China.

    CORRESPONDENCE

    Junyi Ma

    College of Life Science, Northwest Normal University, China.

    skymjy@nwnu.edu.cn

    Received: 14-06-2020; Revised: 15-07-2020; Accepted: 24-02-2021.

    Volume 17, Issue 74 · pp. 334–341 · PUBLISHED 12 July 2021 · DOI: 10.4103/pm.pm_251_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The objective of this study was to examine the antitumor effect and primary mechanisms of LH on cell proliferation, cell cycle, and apoptosis in HepG2 cells. Materials and Methods: The cell viability and proliferation were assessed using Cell Counting Kit‑8 and 5’‑ethynyl‑2’‑deoxyuridine assay. The apoptosis morphological feature of cell was detected with the 4’,6-diamidino-2- phenylindole (DAPI) staining method. The effect of protein expression levels was recognized by Western blot assay. Cell cycle and apoptosis were estimated using flow cytometer. Results: LH repressed cell viability and proliferation of HepG2 cells and persuaded apoptosis in a dose‑dependent way. Flow cytometry analysis results display that LH could arrest cell cycle of HepG2 cells in S phase, thereby preventing cells entering G2/M phase. LH upregulated the expression of cytochrome C, Bax, P53, cleaved caspase‑3, cleaved caspase‑9, and cleaved poly ADP-ribose polymerase (PARP) and suppressed the expression of Bcl‑2. Furthermore, caspase inhibitor z‑VAD‑fmk inhibited the activation of cleaved caspase‑3 and cleaved caspase‑9. Moreover, LH abridged the phosphorylation levels of extracellular signal‑regulated kinase and augmented the phosphorylation levels of c‑Jun N‑terminal kinase and P38. Conclusion: LH designated antitumor effect against HepG2 cells through suppressing cell proliferation, inducing apoptosis and cell cycle arrest by aiming mitogen‑activated protein kinase signaling pathway.

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      Song, N., Ma, J., Zhang, X., Qu, D., Hui, L., Sang, C., & Li, H. (2021). Lappaconitine Hydrochloride Induces Apoptosis and S Phase Cell Cycle Arrest through MAPK Signaling Pathway in Human Liver Cancer HepG2 Cells. Pharmacognosy Magazine, 17(74), 334–341. https://doi.org/10.4103/pm.pm_251_20