Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Comparison of Seven Chemical Components in Callicarpa nudiflora from Different Regions by Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry and its Analgesic Effect

    Yunlu Peng2, Yuanyan Hu3, Lin Sheng2,4,5, Jianping Tian2,4,5★, Jianlei Zhang6★ Corresponding author

    1. 1School of Pharmacy, Hainan Medical University, China.
    2. 2College of Ocean , Hainan University, China.
    3. 3Hainan Key Laboratory for Research and Development of Tropical Herbs, China.
    4. 4Key Laboratory of Tropical Translational Medicine of Ministry of Education, Haikou, China.
    5. 5Qingdao Scitech Innovation Quality Testing Co.Ltd., Qingdao, China.

    CORRESPONDENCE

    Jianping Tian

    College of Ocean , Hainan University, China.

    hy0207060@hainmc.edu.cn

    Received: 17-08-2020; Revised: 12-10-2020; Accepted: 12-03-2021.

    Volume 17, Issue 75 · pp. 468–474 · PUBLISHED 11 November 2021 · DOI: 10.4103/pm.pm_365_20

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: This article focused on the differences in the contents of seven active chemical constituents in C. nudiflora grown in different regions. Moreover, we examined the analgesic effect of C. nudiflora from five arbitrarily designated regions and studied the relationship between the compound contents and analgesic effect. Materials and Methods: A simple and delicate qualitative tandem liquid chromatography quadrupole time of flight mass spectrometry method was first established for the immediate determination of seven active components (luteolin, apigenin, quercetin, oleanolic acid, ursolic acid, protocatechuic acid, and caffeic acid) in C. nudiflora. Ethanol extracts were prepared from the mature leaves of C. nudiflora from five regions and the writhing test was performed by intragastric administration in mice. SPSS 13.0 and Graph Pad Prism 8.01 software were used to analyze the correlation of all data. Results: Calibration curves presented satisfactory linearity, with correlation coefficients >0.99 for all compounds within the concentration range. The compound contents were uppermost in plants from Hainan Province. The contents of the seven active chemical components inclined in the order of caffeic acid > luteolin > apigenin > oleanolic acid > ursolic acid > protocatechuic acid > quercetin. Pharmacological studies showed that C. nudiflora from all five regions had obvious analgesic effects. Conclusion: These results might be helpful for the screening and cultivation of C. nudiflora and its realistic clinical application.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Peng, Y., Hu, Y., Sheng, L., Tian, J., & Zhang, J. (2021). Comparison of Seven Chemical Components in Callicarpa nudiflora from Different Regions by Liquid Chromatography Quadrupole Time of Flight Mass Spectrometry and its Analgesic Effect. Pharmacognosy Magazine, 17(75), 468–474. https://doi.org/10.4103/pm.pm_365_20