Curcumin Mediates the Proliferation and Apoptosis of Colorectal Cancer Cells by Downregulating the Expression of Interleukin‑1β through the Nuclear Factor‑κB Signaling Pathway
Xiaowu Qian1★, Chun Jiang1, Zhengtai Zhu1, Gaohua Han2, Ruixing Wang1, Changhe Zhang3★★ Corresponding author
- 1Departments of Geriatrics, Hospital, Taizhou 225300, Jiangsu, China.
- 2Oncology and, China.
- 3General Surgery, Taizhou People’s Hospital, Taizhou 225300, Jiangsu, China.
CORRESPONDENCE
Xiaowu Qian
Departments of Geriatrics, Hospital, Taizhou 225300, Jiangsu, China.
Received: 27-10-2019; Revised: 06-12-2019; Accepted: 03-12-2020.
Volume 17, Issue 75 · pp. 539–544 · PUBLISHED 11 November 2021 · DOI: 10.4103/pm.pm_388_19
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Objective: In this study, we aimed to elucidate the protective effects and mechanism of interleukin (IL)‑1 β on curcumin‑induced apoptosis in SW480 cells. Materials and Methods: Expression levels of IL‑1 β in CRC tissues and cells were detected by the quantitative reverse transcription polymerase chain reaction and Western blot assays. Followed by the incubation of cells with curcumin, the effect on IL‑1 β was measured. Moreover, after transfection, the effects of IL‑1 β on curcumin‑induced SW480 cellular processes were analyzed by cell counting kit‑8 and flow cytometric analysis. Results: According to the results of this study, IL‑1 β was significantly increased in CRC tissues and cells. However, after incubation of the cells with curcumin, IL‑1 β was downregulated and overexpression of IL‑1 β counteracted the antitumor functions of curcumin in SW480 cells. Further studies have shown that curcumin could promote apoptosis of SW480 cells by inhibiting nuclear factor‑κB (NF‑κB) signaling pathway. Conclusion: Our study validated that curcumin inhibits SW480 cell proliferation but promotes apoptosis by downregulating the expression of IL‑1 β probably through NF‑κB signaling pathway. IL‑1 β may an important target for the treatment of CRC.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Qian, X., Jiang, C., Zhu, Z., Han, G., Wang, R., & Zhang, C. (2021). Curcumin Mediates the Proliferation and Apoptosis of Colorectal Cancer Cells by Downregulating the Expression of Interleukin‑1β through the Nuclear Factor‑κB Signaling Pathway. Pharmacognosy Magazine, 17(75), 539–544. https://doi.org/10.4103/pm.pm_388_19