Antimicrobial Activity, Bioactive Constituents, and Functional Groups in Aqueous Methanol Extract of Polyalthia longifolia (Sonn.) Thwaites Leaves
Feyisara Banji Adaramola2, Roger Murugas Cooposamy3, Olufunmiso Olusola Olajuyigbe3,4★★ Corresponding author
- 1Department of Basic Sciences, Chemistry Unit, School of Science and Technology, Babcock University, Ilisan Remo, Ogun State, Nigeria.
- 2Department of Nature Conservation, Faculty of Natural Sciences, Mangosuthu University of Technology, Durban, South Africa.
- 3Department of Microbiology, School of Science and Technology, Babcock University, Ilisan Remo, Ogun State, Nigeria.
CORRESPONDENCE
Olufunmiso Olusola Olajuyigbe
Department of Microbiology, School of Science and Technology, Babcock University, Ilisan Remo, Ogun State, Nigeria.
Received: 03-03-2020; Revised: 15-04-2020; Accepted: 15-07-2020.
Volume 17, Issue 75 · pp. 594–604 · PUBLISHED 11 November 2021 · DOI: 10.4103/pm.pm_66_20
View on Pharmacogn. Mag. original site ↗
ABSTRACT
Objectives: In this study, the functional groups and bioactive compounds in aqueous methanol extract of P. longifolia leaves and its antimicrobial potential were investigated. Materials and Methods: Bioactive constituents of the extract were assayed with gas chromatography‑mass spectrometry (GC‑MS) and functional groups by Fourier transform infra‑red (FT‑IR), while its phytochemical screening and antimicrobial activity were investigated in vitro using standard protocols. Results: Qualitative phytochemical screening showed the presence of therapeutically important phytochemicals, namely, alkaloids, steroidal and cardiac glycosides, saponins, triterpenes, steroids, phytosterols, resins, phenols, flavonoids, tannins, and diterpenes. The extract showed promising bactericidal and fungicidal properties against the test organisms. Staphylococcus aureus was the most resistant of all the test organisms, while other test organisms were significantly inhibited by the extract in a concentration‑dependent fashion. FT‑IR analysis showed different characteristic peak values confirming the presence of important functional groups, namely, alcohol, alkane, ester, alkene, nitro compounds, amine, carboxylic acid, aromatics, and alkyl halide in the extract. GC‑MS analysis identified 25 bioactive compounds in the leave extract. Some of these compounds include 2‑methoxy‑4‑vinylphenol, copaene, aromadendrene, alpha‑curcumene, caryophyllene oxide, palmitic acid methyl ester, cis‑Z‑alpha‑Bisabolene epoxide, phytol, alpha‑Santoline alcohol, and linolenic acid ethyl ester reportedly possessing antimicrobial, antioxidant, antigenotoxic, antiproliferative, anti‑inflammatory, anticarcinogenic, cardio‑protective, insecticidal, pesticidal, nematicidal, antiandrogenic, antiviral, and analgesic activities. Conclusion: This study underscores the vast therapeutic importance of P. longifolia leaves as an important source of potentially useful bioactive principles.
KEYWORDS
REFERENCES
As publishedShowing references and in-text citations exactly as published.
Cite this article
SELECT FORMAT
Adaramola, F. B., Cooposamy, R. M., & Olajuyigbe, O. O. (2021). Antimicrobial Activity, Bioactive Constituents, and Functional Groups in Aqueous Methanol Extract of Polyalthia longifolia (Sonn.) Thwaites Leaves. Pharmacognosy Magazine, 17(75), 594–604. https://doi.org/10.4103/pm.pm_66_20