Metabolite Profiling and in vitro Evaluation of Lepisanthes fruticosa Fruit Pulp Extract as Inhibitor against Dengue and West Nile Virus NS2B‑NS3 Proteases
Suhaina Supian1★, Machap Chandradevan1, Muhamad Aizuddin Ahmad1, Lina Rozano1, Mohd Shukri Mat Ali1★ Corresponding author
- 1Sanimah Simoh Biotechnology and Nanotechnology Research Centre, Malaysia Agricultural Research and Development Institute, Selangor, Malaysia.
- 2Horticulture Research Centre, Malaysia Agricultural Research and Development Institute, Selangor, Malaysia.
CORRESPONDENCE
Suhaina Supian
Sanimah Simoh Biotechnology and Nanotechnology Research Centre, Malaysia Agricultural Research and Development Institute, Selangor, Malaysia.
Received: 31-03-2021; Revised: 15-04-2021; Accepted: 13-05-2021.
Volume 17, Issue 75 · pp. 636–642 · PUBLISHED 11 November 2021 · DOI: 10.4103/pm.pm_113_21
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ABSTRACT
Objectives: To evaluate the inhibitory activity of Lepisanthes fruticosa pulp extract against NS2B‑NS3 proteases from DENV2 and WNV and identify the metabolites from this fruit extract. Materials and Methods: In vitro DENV2 and WNV NS2B‑NS3 proteases assays were carried out using the methanolic extract of L. fruticosa pulp. Liquid chromatography‑electron spray ionization‑mass spectrometry/mass spectrometry (LC‑ESI‑MS/MS) and gas chromatography‑mass spectrometry/mass spectrometry (GC‑MS/MS) were performed to determine the metabolites present in this fruit species extract. Results: L. fruticosa extract exhibited inhibitory activity toward DENV2 and WNV NS2B‑NS3 proteases with 50% inhibitory concentration value of 1.733 ± 0.195 and 9.245 ± 0.938 mg/mL, respectively. LC‑ESI‑MS/MS of L. fruticosa extract identified epigallocatechin‑catechin, epigallocatechin, epicatechin, catechin, cyanidin rutinoside, procyanidin trimer, rutin, myricetin rhamnohexoside, luteolin glucoside and its derivative which were from the flavonoid group. In addition, GC‑MS/MS identified fatty acids and sterols. Conclusion: The inhibitory activity of L. fruticosa pulp extract toward NS2B‑NS3 proteases from DENV2 and WNV suggests this fruit species as a potential source for the development of antiviral. Metabolites from the groups of flavonols, flavones, and sterols identified in L. fruticosa pulp may contribute to the inhibitory properties of L. fruticosa.
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Supian, S., Chandradevan, M., Ahmad, M. A., Rozano, L., & Ali, M. S. M. (2021). Metabolite Profiling and in vitro Evaluation of Lepisanthes fruticosa Fruit Pulp Extract as Inhibitor against Dengue and West Nile Virus NS2B‑NS3 Proteases. Pharmacognosy Magazine, 17(75), 636–642. https://doi.org/10.4103/pm.pm_113_21