Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Quality Control of Bacopa monnieri by High‑Performance Liquid Chromatography Fingerprinting Combined with Chemometric Methods

    Bhuban Mohan Padhiari1★, Asit Ray1★, Tarun Halder2, Sudipta Jena1, Bibhuti Bhusan Champati1, Ambika Sahoo1, Basudeba Kar1, Biswajit Ghosh2, Pratap Chandra Panda1, Sanghamitra Nayak1★★ Corresponding author

    1. 1Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India.
    2. 2Department of Botany, Ramakrishna Mission Vivekananda Centenary College, Rahara, Kolkata, West Bengal, India.

    CORRESPONDENCE

    Bhuban Mohan Padhiari

    Centre for Biotechnology, Siksha O Anusandhan (Deemed to be University), Bhubaneswar, Odisha, India.

    sanghamitran24@gmail.com

    Received: 04-03-2021; Revised: 27-07-2021; Accepted: 16-11-2021.

    Volume 17, Issue 76 · pp. 872–881 · PUBLISHED 24 January 2022 · DOI: 10.4103/pm.pm_107_21

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Objectives: The present work was carried out to develop the chemical fingerprint and quality consistency of B. monnieri. Materials and Methods: Twenty‑four accessions of B. monnieri were collected from two provinces of Eastern India (Odisha and West Bengal) and were evaluated by high‑performance liquid chromatography (HPLC) technique by selecting twelve common peaks. In addition, the method was validated in terms of precision, stability, repeatability, and recovery test. Moreover, chemometric analyses such as hierarchical cluster analysis, principal component analysis, and discriminant analysis were constructed from chemical fingerprint data to classify sample of different geographical origins. Results: The similarities analysis values were within the range of 0.81%–0.98%. In quantitative analysis, eight analytes displayed acceptable regression (R2 > 0.989%) within the test range. The results of chemometrics were in agreement and grouped B. monnieri samples into two groups in accordance with the geographical origin. Bacopaside II, bacopaside I, and bacoside A3 are the influencing variable responsible for differentiating B. monnieri samples from different regions. Conclusion: The results suggested that HPLC fingerprint in combination with chemometric techniques and quantitative analysis could be used for differentiation and assessing the quality consistency of B. monnieri.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Padhiari, B. M., Ray, A., Halder, T., Jena, S., Champati, B. B., Sahoo, A., Kar, B., Ghosh, B., Panda, P. C., & Nayak, S. (2022). Quality Control of Bacopa monnieri by High‑Performance Liquid Chromatography Fingerprinting Combined with Chemometric Methods. Pharmacognosy Magazine, 17(76), 872–881. https://doi.org/10.4103/pm.pm_107_21