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    Anticancer Effect of Benzyl Isothiocyanate on the Apoptosis of Human Gemcitabine‑Resistant Pancreatic Cancer MIA PaCa‑2/ GemR Cells (MIA RG100)

    Chao‑Wen Hsueh1, Chun Chen2★, Po‑Yen Chiu3, Ni‑Na Chiang4, Chi‑Cheng Lu5, Jai‑Sing Yang6, Fu‑An Chen3★ Corresponding author

    1. 1Department of Internal Medicine, Division of Gastroenterology, Kaohsiung Armed Forces General Hospital, Kaohsiung 802, Taiwan.
    2. 2School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.
    3. 3Department of Pharmacy and Master Program, Tajen University, Pingtung 907, Taiwan.
    4. 4Kaohsiung Veterans General Hospital, Kaohsiung 813, Taiwan.
    5. 5Department of Sport Performance, National Taiwan University of Sport, Taichung 404, Taiwan.
    6. 6Department of Medical Research, China Medical University Hospital, China Medical University, Taichung 404, Taiwan.

    CORRESPONDENCE

    Chun Chen

    School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 807, Taiwan.

    fachen@tajen.edu.tw

    Received: 25-02-2022; Revised: 30-05-2022; Accepted: 12-07-2022.

    Volume 18, Issue 79 · pp. 675–678 · PUBLISHED 19 September 2022 · DOI: 10.4103/pm.pm_101_22

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Benzyl isothiocyanate (BITC) is a natural compound found in numerous cruciferous vegetables, and research has indicated that it has diverse biological activities. Isothiocyanate and its derivatives are the major anticancer natural compounds in cruciferous vegetables; these compounds help inhibit tumor cell proliferation through various mechanisms such as promoting tumor cell apoptosis, prompting cycle arrest, and increasing the generation of reactive oxygen species (ROS).Objectives: In human pancreatic cancer, gemcitabine is the first‑line treatment; however, pancreatic cancer cells readily develop resistance to gemcitabine. Studies have demonstrated that natural products can promote the effect of gemcitabine and enhance the apoptosis process; however, the relevant mechanism and potential of BITC in human pancreatic cancer cells with gemcitabine resistance, namely, MIA PaCa‑2/GemR cells (MIA RG100), are unclear. Materials and Methods: To elucidate the extent to which BITC induces apoptosis, we investigated the time and dose‑dependent cell viability of PaCa‑2/GemR cells under treatment with BITC. Results: Following BITC treatment, the PaCa‑2/GemR cells exhibited DNA condensation, as indicated by transferase‑mediated d‑UTP nick end labeling (TUNEL) stain, with a corresponding increase in ROS production in mitochondria. Moreover, colorimetric assay analyses revealed that BITC increased caspase‑9 and caspase‑3 activities in PaCa‑2/GemR cells. Our results indicate that BITC induces apoptotic cell death in PaCa‑2/GemR cells through a mitochondrial‑dependent signaling pathway.

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      Hsueh, C., Chen, C., Chiu, P., Chiang, N., Lu, C., Yang, J., & Chen, F. (2022). Anticancer Effect of Benzyl Isothiocyanate on the Apoptosis of Human Gemcitabine‑Resistant Pancreatic Cancer MIA PaCa‑2/ GemR Cells (MIA RG100). Pharmacognosy Magazine, 18(79), 675–678. https://doi.org/10.4103/pm.pm_101_22