Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Study on Chemical Constituents of the Enriched Extracts of Erigeron multiradiatus and Anti-thrombotic Effect on Zebrafish

    Li Li1, Wenfang Jin1, Yue Wang1, Li Yang1, Zhi-Feng Zhang1★★ Corresponding author

    1. 1Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory, Southwest Minzu University, Chengdu, Sichuan, China.

    CORRESPONDENCE

    Zhi-Feng Zhang

    Sichuan Provincial Qiang-Yi Medicinal Resources Protection and Utilization Technology Engineering Laboratory, Southwest Minzu University, Chengdu 610041, Sichuan, China.

    zfzhang@swun.edu.cn

    Received: 27-08-2022; Accepted: 07-11-2022.

    Volume 19, Issue 3 · pp. 638–650 · PUBLISHED 2023 · DOI: 10.1177/09731296231184536

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Erigeron multiradiatus (Lindl.) Benth. has been used for years to treat various diseases in Traditional Tibetan Medicine (TTM). Our previous studies have shown that E. multiradiatus extract has important pharmacological effects, such as anti-myocardial ischemia–reperfusion (I/R) injury, anti-inflammatory, and anti-diabetic effects. To date, there have been no reports on using this extract to treat thrombosis. Objectives: The aim of this study was to enrich the active components of E. multiradiatus and to further investigate its anti-thrombotic activity. Materials and Methods: We used D101 macroporous adsorption resin to enrich the main active substances of E. multiradiatus and performed quantitative determination using ultra-high-performance liquid chromatography with a photodiode array detector (UHPLC-PDA). Furthermore, we investigated the anti-thrombotic effects of E. multiradiatus using three models: an arachidonic acid-induced platelet aggregation thrombotic zebrafish model, a phenylhydrazine-induced red blood cell (RBC)- injuring thrombotic zebrafish model, and a ponatinib-induced vascular endothelial cell (VEC)-injuring thrombotic zebrafish model. Results: The yield of active component enrichment of E. multiradiatus was 4.95% using the D101 resin. We accurately determined eight compounds in the extract. Our results showed that these extracts had significant anti-thrombotic effects in the three different zebrafish models. Conclusion: The enriched extract of E. multiradiatus could be used as a natural anti-thrombotic agent. Our results will provide a reference for reasonable clinical applications of E. multiradiatus.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Li, L., Jin, W., Wang, Y., Yang, L., & Zhang, Z. (2023). Study on Chemical Constituents of the Enriched Extracts of Erigeron multiradiatus and Anti-thrombotic Effect on Zebrafish. Pharmacognosy Magazine, 19(3), 638–650. https://doi.org/10.1177/09731296231184536