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    Article

    Obtaining Acid-sensitive Prosaikogenin F by Enzymatic Hydrolysis of Saikosaponin A

    Yeting Zhu1, Jiangsong Peng1, Yaqin Zhao1, Mengru Wu1, Suping Chen1, Jiali Shao1, Xubo Wang2, Guohua Xia1★, Yuping Shen1★ Corresponding author

    1. 1Department of Chinese Materia Medica and Pharmacy, School of Pharmacy, Jiangsu University, Jiangsu, China.
    2. 2Department of Chemical Engineering and Pharmaceutical Engineering, Changzhou Vocational Institute of Engineering, Jiangsu, China.

    CORRESPONDENCE

    Guohua Xia

    Department of Chinese Materia Medica and Pharmacy, School of Pharmacy, Jiangsu University, 301 Xuefu Road, Zhenjiang, Jiangsu 212013, China.

    xgh-78131@yeah.net

    Received: 24-04-2022; Accepted: 26-10-2022.

    Volume 19, Issue 3 · pp. 689–699 · PUBLISHED 2023 · DOI: 10.1177/09731296231169577

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Prosaikogenin F (PSF) has stronger anti-cancer bioactivity than saikosaponin A (SSA), however, it was hardly isolated due to its trace amount in the raw material of Radix Bupleuri (RB). In addition, the active chemical constituent was unstable under acidic conditions owing to a 13,28-epoxy-ether moiety at the D ring of its aglycone. Objectives: This study was to develop an appropriate method for obtaining acid-sensitive PSF from SSA abundant in RB. Materials and Methods: Enzymatic hydrolysis was employed and snailase was selected due to its good hydrolysis performance under nearly neutral circumstances. Hydrolysis conditions were then optimized by one-factor-at-a-time experimentation before response surface methodology (RSM) by Box-Behnken Design (BBD). Results: The reaction system was constructed in Na2HPO4-NaH2PO4 buffer (pH 6.0) containing snailase/SSA (44:1) at 39°C, then the hydrolysis lasted for 12 h. Therefore, the highest conversion ratio of SSA was achieved at 100.0%. Conclusion: The newly proposed method is eco-friendly for obtaining acid-sensitive PSF, which lays a solid foundation for its development to be an anti-cancer new drug.

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      Zhu, Y., Peng, J., Zhao, Y., Wu, M., Chen, S., Shao, J., Wang, X., Xia, G., & Shen, Y. (2023). Obtaining Acid-sensitive Prosaikogenin F by Enzymatic Hydrolysis of Saikosaponin A. Pharmacognosy Magazine, 19(3), 689–699. https://doi.org/10.1177/09731296231169577