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    Article

    Corilagin Induces ROS-mediated Apoptosis and Triggers Cell-Cycle Arrest at G0/G1 Stage in Osteosarcoma Cells

    Jichao Liu1, Dong Yang2, Jianyu Liu3★★ Corresponding author

    1. 1Department of Hand and Foot Micro Burn Plastic Surgery, 3201 Hospital, Hanzhong, China
    2. 2Department of Joint Surgery, The Third Hospital of Jinan, Jinan, China
    3. 3Department of Trauma Orthopedics, Shandong Second Provincial General Hospital, Jinan, China

    CORRESPONDENCE

    Jianyu Liu

    Department of Trauma Orthopedics, Shandong Second Provincial General Hospital, Jinan 50000, China.

    ac33800@sina.com

    Received: 27-12-2021; Accepted: 13-10-2022.

    Volume 19, Issue 4 · pp. 853–861 · PUBLISHED 2023 · DOI: 10.1177/09731296231184539

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Osteosarcoma (OS) is an extremely aggressive primary bone cancer (BC) malignancy. A variety of malignancies can develop in the bones, including BC. Primary BCs are tumors that start in the bone. Bones can also become affected by tumors that start in the body’s organs or other tissues. Objectives: Several reports suggested that corilagin (CL) exerts anticancer properties on various kinds of tumor cells; however, its molecular action on OS cells remains undefined. Materials and Methods: The CL activity of OS cells’ cytotoxicity, intracellular reactive oxygen species (ROS), mitochondrial membrane potential (MMP), apoptosis, and cell-cycle distribution was evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay, dichloro-dihydro-fluorescein diacetate (DCFH-DA), Rh-123, acridine orange and ethidium bromide (AO/EB), 4′,6-diamidino-2-phenylindole (DAPI), and flow cytometry analysis. Results: Results revealed that CL could suppress OS cell proliferation via enhanced intracellular ROS, and MMP loss, and triggered apoptosis in a dose-dependent has an inferior manner. Our findings demonstrated that CL alleviates U2OS and MG-63 cell proliferation by the ROS-mediated apoptosis, which triggers G0/G1 cell-cycle arrest. Conclusion: Thus, CL might be a protective therapeutic agent against BC.

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      Liu, J., Yang, D., & Liu, J. (2023). Corilagin Induces ROS-mediated Apoptosis and Triggers Cell-Cycle Arrest at G0/G1 Stage in Osteosarcoma Cells. Pharmacognosy Magazine, 19(4), 853–861. https://doi.org/10.1177/09731296231184539