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    Induction of Apoptosis by Cynaropicrin in Human Colon Cancer Cell Line HCT-116 through the Mitochondria-mediated Apoptotic Pathway

    Lei Wang1, Xiaoqin Bie2, Suresh Mickymaray3,4, Abdulaziz S. Alothaim3, Yan Pei1, Huaping Gong5★★ Corresponding author

    1. 1Department of Gastrointestinal Surgery, The Fifth Affiliated Hospital of Xinjiang Medical University, Urumqi, Xinjiang, China
    2. 2Department of Gastroenterology, Huai’an Affiliated Hospital of Xuzhou Medical University, Huaian, Jiangsu, China
    3. 3Department of Biology, College of Science, Al-Zulfi, Majmaah University, Majmaah, Saudi Arabia
    4. 4Centre of Molecular Medicine and Diagnostics (COMManD), Saveetha Dental College and Hospitals, Saveetha Institute of Medical and Technical Sciences, Saveetha University, Chennai, Tamil Nadu, India
    5. 5First Clinical College, Renmin Hospital of Wuhan University, Wuhan, Hubei, China

    CORRESPONDENCE

    Huaping Gong

    First Clinical College, Renmin Hospital of Wuhan University, Wuhan, Hubei 430060, China.

    gonghuaping147@outlook.com

    Received: 16-08-2022; Accepted: 13-10-2022.

    Volume 19, Issue 4 · pp. 874–884 · PUBLISHED 2023 · DOI: 10.1177/09731296231183833

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Colon cancer continues to be the third most commonly occurring cancer around the globe with both lifestyle-related risk factors and genetic dispositions. Owing to the increased incidence of the disease, there is a need to find new treatment options which are easily accessible and less toxic to the cells. Cynaropicrin, a sesquiterpene extract from the artichoke plant exhibits varied properties such as anti-inflammatory, anti cancer, and anti oxidant among others. In the current investigation, the anti-cancer potential of the cynaropicrin extract was observed against colon cancer in HCT-116 cell lines. Materials and Methods: The drug exhibited a considerable decrease in cell proliferation. Also analyzed was the reactive oxygen species (ROS) generation and apoptosis induction by cynaropicrin on the HCT-116 cell line. The AO/EtBr staining confirmed the apoptotic induction through chromatin condensation by fluorescence microscopic examination followed by DAPI staining of the treated HCT-116 cells which showed nuclear condensation and disintegration. Results: Furthermore, it was observed that cynaropicrin elevated the levels of ROS in the cells which modified the mitochondrial membrane permeability in the HCT-116 cell lines. The study also evaluated the levels of apoptotic proteins and the expression of inflammatory cytokines concerning cells treated with cynaropicrin and untreated control cells. Conclusion: It has been observed that cynaropicrin treatment can lead to apoptosis in the HCT-116 cell line via mitochondria-mediated apoptotic pathway and if further evaluated, it can turn out to be a potent anti-cancer drug for effective management of colon cancer.

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      Wang, L., Bie, X., Mickymaray, S., Alothaim, A. S., Pei, Y., & Gong, H. (2023). Induction of Apoptosis by Cynaropicrin in Human Colon Cancer Cell Line HCT-116 through the Mitochondria-mediated Apoptotic Pathway. Pharmacognosy Magazine, 19(4), 874–884. https://doi.org/10.1177/09731296231183833