Comparative Chemical Profiling of a Polyherbal Formulation with Respect to its Ingredient Plants Using Spectrophotometric and High-performance Thin-layer Chromatographic Techniques

ABSTRACT

Background: Guggulutiktam Kashayam (GTK) is a water decoction used in Ayurveda for the treatment of various diseases like skin diseases, ulcers, anemia, thyroid problems, stress, liver disease, and leucorrhoea. It is a polyherbal formulation prepared from specific parts of 28 medicinal plants. Objectives: The study aimed at a comparative phytochemical evaluation of an important polyherbal formulation with its ingredient plants. Materials and Methods: Chemical profiling of GTK has been carried out in comparison with its ingredient plants. Spectrophotometric estimation of major class compounds and high-performance thin-layer chromatography (HPTLC) profiling were done using a standardized protocol. Results: The results of the study showed that the phytochemical contents of the finished formulation are different from those of the ingredient plants, which might be due to the numerous synergistic and antagonistic interactions of numerous chemical components extracted from different ingredient drugs. HPTLC-based comparative chromatograms showed the differences and similarities in certain compounds that are specific to some of the plant ingredients. Conclusion: The study established the cumulative chemical interactions of various phytochemicals during the preparation of an important polyherbal formula used in Ayurveda.

KEYWORDS

• Chemical profiling
• polyherbal formulation
• phytochemistry
• HPTLC

Showing references and in-text citations exactly as published.

• DOI10.1177/09731296231200365Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Mag..

• Article · VOLUME 19, ISSUE 4Effect of Ligustrazine on Lipopolysaccharide-induced Inflammatory Response of Vascular Endothelial Cells and its Possible MechanismXia Meng Zhang, Zehong Ma, Chengjian Mao, Jihong Zhang, Zhongdanni Ma · pp. 994–1002Original ArticleSame type: Article
• Article · VOLUME 19, ISSUE 4In vitro Neuroprotective Activity of Vigna radiata L. and Vigna pilosa L. on Amyloid Beta-induced CytotoxicityN. V. Prasanth, P. Pandian, T. Balasubramanian · pp. 1003–1011Original ArticleSame type: Article
• Article · VOLUME 19, ISSUE 4Voacangine Mitigates Human Nasopharyngeal Carcinoma Cells HK-1 Proliferation and Triggers Apoptosis through the Suppression of NF-κB Facilitated PI3K/AKT/mTOR PathwayAihui Hou, Huimin Zhen, Xiaofeng Qiao, Herong Dang, Yuanfeng Shen · pp. 1012–1020Original ArticleSame type: Article
• Article · VOLUME 19, ISSUE 4Scutellarin Protects Myocardial Ischemia-Reperfusion Injury ERK1/2-CREB Regulated MitophagyWei-Wei Wang, Xiao-Lei Liu, Yu Ding, Ya-Di Chen, Dan Ma, Rui Bu, Tian-Hao Bao · pp. 1021–1036Original ArticleSame type: Article