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    Phytochemical Composition, Bioavailability and Pharmacokinetics of Scorzonera undulata Methanolic Extracts: Antioxidant, Anticancer, and Apoptotic Effects on MCF7 Cells

    Imen Bédoui1, Hmed Ben Nasr1, Kamilia Ksouda1, Wajdi Ayadi2, Nour Louati3, Mohamed Chamkha4, Sirine Choura4, Jalel Gargouri3, Serria Hammami1, Hanen Affes1, Walid S. Hamadou5, Nourhene Zammel6, Subuhi Sherwani5, Khaled Zeghal1, Riadh Badraoui5,6,7★★ Corresponding author

    1. 1Laboratory of Pharmacology, Faculty of Medicine of Sfax, University of Sfax, Sfax, TUNISIA.
    2. 2Laboratory of Molecular Biotechnology of Eukaryotes, Centre of Biotechnology of Sfax, Sfax, TUNISIA.
    3. 3Regional Blood Transfusion Center of Sfax, Faculty of Medicine of Sfax, University of Sfax, Sfax, TUNISIA.
    4. 4Laboratory of Environmental Bioprocesses, Centre of Biotechnology of Sfax, University of Sfax, Sfax, TUNISIA.
    5. 5Laboratory of General Biology, College of Science, University of Ha’il, Ha’il, SAUDI ARABIA.
    6. 6Section of Histology-Cytology, Faculty of Medicine of Tunis, University of Tunis El Manar, La Rabta-Tunis, TUNISIA.
    7. 7Laboratory of Histo-Embryology and Cytogenetics, Faculty of Medicine of Sfax, University of Sfax, Sfax, TUNISIA.

    CORRESPONDENCE

    Riadh Badraoui

    riadh.badraoui@fmt.utm.tn

    Received: 07-05-2023; Accepted: 03-08-2023.

    Volume 20, Issue 1 · pp. 218–229 · PUBLISHED 2024 · DOI: 10.1177/09731296231207231

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Finding new effective substances to develop drugs against cancer or to prevent it remains a big challenge facing scientists and clinicians. Objectives: In such an issue, we tested the anti-tumoral effect of Scorzonera undulata extracts on MCF7 cells. Materials and Methods: Methanolic extracts of S. undulata roots (RSU) and aerial parts (ASU) from the Ha’il region were analyzed by HPLC. Their DPPH scavenging, ferric-reducing antioxidant power (FRAP) and total antioxidant potentials were evaluated. The MTT test was used to determine their cytotoxic effects on MCF7 cells using different concentrations, and their potential to induce apoptosis was determined by measuring the mitochondrial transmembrane loss of potential by flow cytometry using the JC-1 fluorescent marker. Results: S. undulata was found to be rich in flavonoids, tannins, and polyphenols. ASU contains great amounts of apigenin and gallic acid, whereas RSU contains luteolin and chlorogenic acid as major components. The potential to scavenge DPPH and the total antioxidant activity of ASU were twofold greater than those of RSU. MCF7 cell viability was reduced by 50% at concentrations of 4.22 ± 0.06 and 5.89 ± 0.08 mg.mL−1, respectively, for ASU and RSU at 24 h. In addition, S. undulata extracts induced MCF7 cells’ death through cell lysis and apoptosis. Conclusion: As an edible vegetable, S. undulata is worth further exploration in alternative and complementary medication as it possesses significant antioxidant and anti-cancer potentials.

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      Bédoui, I., Nasr, H. B., Ksouda, K., Ayadi, W., Louati, N., Chamkha, M., Choura, S., Gargouri, J., Hammami, S., Affes, H., Hamadou, W. S., Zammel, N., Sherwani, S., Zeghal, K., & Badraoui, R. (2024). Phytochemical Composition, Bioavailability and Pharmacokinetics of Scorzonera undulata Methanolic Extracts: Antioxidant, Anticancer, and Apoptotic Effects on MCF7 Cells. Pharmacognosy Magazine, 20(1), 218–229. https://doi.org/10.1177/09731296231207231