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    Pharmacognosy MagazineNOW IN
    Article

    Mechanism of Ziyuglycoside II-mediated Ferroptosis-related Proteins on the Proliferation and Metastasis of Human Lung Adenocarcinoma Cell Lines

    Jian-hui Zhang1, Li-jun Xie2, Han-lu Wang1, Hui Chen1, Xiao-rong Meng1,3, Xing Lin1,4, Xin-fu Lin1,5, Li-sheng Liao1,6, Ting Chen1, Jie-Wei Luo1,3★, Lu-yu Hong2, Xin Chen1,7★ Corresponding author

    1. 1Department of Pathology, Fujian Provincial Hospital, Fuzhou, CHINA.
    2. 2Department of Hematology, Fujian Provincial Hospital, Fuzhou, CHINA.
    3. 3Department of Pediatrics, Fujian Provincial Hospital, Fuzhou, CHINA.
    4. 4Department of Traditional Chinese Medicine, Fujian Provincial Hospital, Fuzhou, CHINA.
    5. 5Department of Oncology of Zhangzhou Traditional Chinese Medicine Hospital, Zhangzhou, CHINA.
    6. 6Shengli Clinical Medical College of Fujian Medical University, Fuzhou, CHINA.
    7. 7Department of Thoracic Surgery, Fujian Provincial Hospital, Fuzhou, CHINA.

    CORRESPONDENCE

    Jie-Wei Luo

    Department of Pathology, Fujian Provincial Hospital, Fuzhou, CHINA.

    docluo0421@aliyun.com

    Received: 06-08-2022; Accepted: 26-10-2022.

    Volume 20, Issue 1 · pp. 7–15 · PUBLISHED 2024 · DOI: 10.1177/09731296231169588

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Ferroptosis is a novel type of regulated cell death and targeting ferroptosis may be a potential treatment strategy for lung cancer. Ziyuglycoside II (ZYG II) has a significant inhibitory effect on the growth of lung cancer cells. However, the selective anti-tumor effect of the ZYG II against lung cancer has not been systemically studied. Objectives: We combined ferrostatin-1 and erastin to explore the potential therapeutic mechanism of the ZYG II for lung adenocarcinoma. Materials and Methods: A549 and H1299 cells were randomly divided into the control, ZYG II, ferroptosis inhibitor group (ZYG II+ ferrostatin-1), and erastin group (ZYG II+ erastin). Cell proliferation was detected using the CCK-8 method. Cell migration and invasion were evaluated using the Transwell assay. The protein expression levels of Glutathione Peroxidase 4 (GPX4), Solute Carrier Family 7 Member 11 (SLC7A11), and Transferrin receptor 1 (TFR1) were measured using western blotting. Results: Compared with the control group, the cell proliferation, migration, and invasion abilities of the ZYG II group significantly decreased, the protein expression levels of GPX4 and SLC7A11 in the ZYG II group declined significantly, and the expression of TFR1 increased significantly (p < 0.05). After adding ferrostatin 1 (ZYG II+ Ferrostatin 1), the cell proliferation, migration, and invasion abilities of the inhibited cells were significantly increased, the expression of GPX4 and SLC7A11 increased significantly and the expression of TFR1 decreased significantly (p < 0.05). However, after adding the erastin (ZYG II+ erastin), the cell viability was further inhibited in A549, the expression levels of GPX4 and SLC7A11 were further inhibited and the expression of TFR1 was further increased (p < 0.05). Conclusion: ZYG II significantly inhibited the survival rate, proliferation, migration, and invasion ability of A549 and H1299 cells, possibly by inducing ferroptosis.

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      Zhang, J., Xie, L., Wang, H., Chen, H., Meng, X., Lin, X., Lin, X., Liao, L., Chen, T., Luo, J., Hong, L., & Chen, X. (2024). Mechanism of Ziyuglycoside II-mediated Ferroptosis-related Proteins on the Proliferation and Metastasis of Human Lung Adenocarcinoma Cell Lines. Pharmacognosy Magazine, 20(1), 7–15. https://doi.org/10.1177/09731296231169588