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    Article

    Lactobacillus plantarum Enhanced the Cardioprotective Efficacy of Arctiin in Isoproterenol Cardiac Injury in BALB/c Mice

    Kun Jiang1, Yanjun Jia2, Tahani Awad Alahmadi3, Samer Hasan Hussein-Al-Ali4, Yajing Wei5★★ Corresponding author

    1. 1Department of Cardiology, Bazhong Central Hospital, Bazhong, Sichuan Province, CHINA.
    2. 2ECG Function Department, Hengshui Eighth People’s Hospital, Hengshui, Hebei Province, CHINA.
    3. 3Department of Pediatrics, College of Medicine and King Khalid University Hospital, King Saud University, Medical City, Riyadh, SAUDI ARABIA.
    4. 4Department of Chemistry, Faculty of Science, Isra University, Amman, JORDAN.
    5. 5Department of Cardiology, Xianyang Central Hospital, Xianyang, Shaanxi Province, CHINA.

    CORRESPONDENCE

    Yajing Wei

    Department of Cardiology, Xianyang Central Hospital, Xianyang, Shaanxi Province, CHINA.

    xinnei123123@sina.com

    Received: 15-06-2023; Accepted: 06-09-2023.

    Volume 20, Issue 2 · pp. 430–443 · PUBLISHED 2024 · DOI: 10.1177/09731296231211453

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Isoproterenol is a drug used for the treatment of bradycardia that has many side effects due to its non-selective β-adrenoceptor agonist properties. Aim: In this study, we evaluated an in vivo experimental model for isoproterenol (ISO) cardiac injury using BALB/c mice and protective treatment with a combined optimized dose of Arctiin (AR) and Lactobacillus plantarum (LP) (ARLP; 30 mg/kg b.w. AR and viable 106 CFU/mL LP p.o.) together. Introduction: Isoproterenol causes cardiac injury in many instances. Arctiin is a naturally occurring lignin glycoside present in many herbal medicinal plants that has many beneficial effects for humans. Lactobacillus plantarum is yet another beneficial probiotic that benefits largely to humans. Materials and Methods: Male BALB/c mice in appropriate groups (6 animals each) were treated with ARLP for 7 days with ISO administration (100 mg/kg i.p.) on days 5 and 6. On the 8th day, animals were sacrificed. Serum and heart samples were collected for further processing. CK-MB, cardiac troponin, AST, ALT, LDH, GST, GPx, CAT, SOD, GSH, GSSG, MDA, nuclear factor kappa B (NFκB), TNF-α, and IL-6 by ELISA and gene expressions of nuclear factor erythroid 2-related factor 2 (Nrf2) were estimated in samples. Results: Mice administered with ISO showed a prominent rise in the serum marker enzyme activities and tissue oxidative stress markers (MDA and GSSG). Furthermore, marked reductions in body weight and enzymatic and non-enzymatic antioxidant levels were noticed in ISO toxicity. Alterations in proinflammatory cytokines (TNF-α and IL-6) and Nrf2 levels by RT-PCR were analyzed. Histopathology of the heart also indicated cardiac injury in ISO-intoxicated mice. ARLP pretreatment prevented all these cardiac injuries and exerted significant (p ≤ 0.05) prophylactic protection toward cardiac tissue. Further, we analyzed the possible interactions between AR and LP in vitro, to understand the influence of ARonLP, which showed no significant suppressive/antibacterial activity on LP. Conclusion: In conclusion, ARLP exerts a strong cardioprotective and anti-inflammatory activity in cardiovascular injury.

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      Jiang, K., Jia, Y., Alahmadi, T. A., Hussein-Al-Ali, S. H., & Wei, Y. (2024). Lactobacillus plantarum Enhanced the Cardioprotective Efficacy of Arctiin in Isoproterenol Cardiac Injury in BALB/c Mice. Pharmacognosy Magazine, 20(2), 430–443. https://doi.org/10.1177/09731296231211453