Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Huanglian Jiedu Decoction Inhibits LPS-induced Neuroinflammation and Attenuates the Activation of NLRP3 Inflammasome in BV2 Cells

    Junli Wang1, Rui Wang2, Yaming Du2, Xinzi Xu2, Xin Liu1, Wei Shao1, Guohua Chen1,2★★ Corresponding author

    1. 1College of Clinical Chinese Medicine, Hubei University of Chinese Medicine, Wuhan, Hubei, China
    2. 2Department of Neurology, Wuhan No. 1 Hospital, Wuhan, Hubei, China

    CORRESPONDENCE

    Guohua Chen

    Department of Neurology, Wuhan No. 1 Hospital, Wuhan, Hubei 430022, China.

    cghys_2008@163.com

    Received: 13-09-2023; Accepted: 06-02-2024.

    Volume 20, Issue 4 · pp. 1359–1366 · PUBLISHED 2024 · DOI: 10.1177/09731296241244575

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Huanglian Jiedu decoction (HLJDD) is a classical herbal formula for treating inflammatory diseases with Traditional Chinese Medicine (TCM). However, the impacts of HLJDD on neuroinflammation and the precise mechanisms involved remain poorly understood. Purpose: In the present study, HLJDD was found to inhibit lipopolysaccharide (LPS) induced neuroinflammatory responses in BV2 microglia via regulating NLRP3 inflammasome activation. Materials and Methods: Anti-inflammatory activity was evaluated with ELISA assay and the polarization changes were observed using immunofluorescent staining. The levels of NLRP3 inflammasome activation were determined using qPCR and Western blotting. Results: The administration of HLJDD successfully countered LPS-induced neuroinflammatory responses by attenuating microglial activation and fostering a phenotypic conversion from the pro-inflammatory M1 to the anti-inflammatory M2. Additionally, HLJDD treatment demonstrated inhibition of NLRP3 inflammasome activation. Conclusion: The findings of the present study may provide insights into how HLJDD could be a therapeutic drug for neuroinflammatory diseases associated with regulating NLRP3 inflammasome and microglial activation.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Wang, J., Wang, R., Du, Y., Xu, X., Liu, X., Shao, W., & Chen, G. (2024). Huanglian Jiedu Decoction Inhibits LPS-induced Neuroinflammation and Attenuates the Activation of NLRP3 Inflammasome in BV2 Cells. Pharmacognosy Magazine, 20(4), 1359–1366. https://doi.org/10.1177/09731296241244575