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    Puerarin Promotes Tibial Shaft Fracture Healing in Rats and its Relationship with BMP-Smad Pathway

    and can further recruit substances such as Smad1, its Relationship with BMP-Smad Pathway Yini Mao1, Jun Li2, Feifei Zhang3★★ Corresponding author

    1. 1Department of Trauma Sports Orthopedics, Hunan Provincial Brain Hospital (Hunan Second People’s Hospital), Changsha, Hunan, CHINA.
    2. 2Department of Neurosurgery, Hunan Provincial Brain Hospital (Hunan Second People’s Hospital), Changsha, Hunan, CHINA.
    3. 3Department of Nursing, Hunan Provincial Brain Hospital (Hunan Second People’s Hospital), Changsha, Hunan, China gene expression, and promote osteoblast differentiation (Caddy et al., 2020; Fu et al., 2020). The BMP-Smad pathway can promote phosphorylation of Smad protein and induce the.

    CORRESPONDENCE

    Feifei Zhang

    Department of Nursing, Hunan Provincial Brain Hospital (Hunan Second People’s Hospital), Changsha, Hunan, China gene expression, and promote osteoblast differentiation (Caddy et al., 2020; Fu et al., 2020). The BMP-Smad pathway can promote phosphorylation of Smad protein and induce the.

    kenxun64644@126.com

    Received: 13-09-2024; Accepted: 06-11-2024.

    Volume 21, Issue 3 · pp. 865–876 · PUBLISHED 2025 · DOI: 10.1177/09731296241305868

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Fracture healing involves multiple growth factors and the interaction between multiple signaling pathways. Pue (puerarin) is a natural compound with multiple biological activities, but its effect on fracture healing and its relationship with the bone morphogenic protein (BMP)-Smad pathway is not yet clear. Objectives: This study intends to assess the effect of puerarin on fracture healing. Materials and Methods: Seventy rats were used for the study to establish a tibial shaft fracture rat model. The experimental rats were divided into a tibial fracture group, Pue dose group (low, medium, and high), BMP-2 group, Noggin group, Pue + SY-LB-35 group, and Pue + Noggin group through the random number method. We observed the fracture healing through micro-CT and histology, observed the tibial stump tissue with hematoxylin and eosin (HE) staining, and detected the expression of genes related to the BMP-Smad pathway through quantitative real-time polymerase chain reaction (qPCR) and Western blot. Results: We found that puerarin can promote tibial shaft fracture healing in rats in a dose-dependent manner; through micro-CT and histological analysis, we observed increased trabecular bone formation during fracture healing in rats treated with puerarin. Callus formation is accelerated, and bone density increases. In addition, puerarin also significantly increased the expression of genes related to the BMP-Smad pathway, including BMP2, Smad1, Smad5, and so on. Conclusion: Puerarin can promote tibial shaft fracture healing in rats, and its mechanism is related to upregulating the expression of genes related to the BMP-Smad pathway. It further improves the fracture-healing mechanism and provides candidate drugs for the treatment of tibial shaft fracture healing in clinical medicine.

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      Smad, A. C. F. R. S. S. A., Mao, I. R. W. B. P. Y., Li, J., & Zhang, F. (2025). Puerarin Promotes Tibial Shaft Fracture Healing in Rats and its Relationship with BMP-Smad Pathway. Pharmacognosy Magazine, 21(3), 865–876. https://doi.org/10.1177/09731296241305868