Eucalyptol Attenuates Cerebral Ischemia/Reperfusion Injury in Mice

ABSTRACT

Background: One of the leading causes of acute brain injury is cerebral ischemia/reperfusion (I/R) injury. It is an ailment that occurs when the brain does not receive enough oxygen-rich blood supply, which damages brain cells. Oxidative stress is recognized as a significant mechanism that causes I/R injury and is a critical focal point for therapy. Apoptosis and inflammation are all pathogenic processes underpinning I/R damage. Eucalyptol (EU), a plant-derived substance, protects neuronal damage. We postulate that EU inhibits apoptosis and oxidative stress after focal cerebral I/R damage in mice. Objectives: Thus, the present work sought to examine the neurological protective benefits of EU in mice models of cerebral ischemia/reperfusion (CI/R) injury. Materials and Methods: The measurement of neurological function, infarct volume, and the amount of water in the brain were evaluated subsequently. The brain tissue was examined to assess the activities of oxidative stress, inflammatory cytokines, and matrix metalloproteinases (MMP) levels, and histopathology was determined. Using enzyme-linked immunosorbent assay (ELISA), apoptotic marker expression was used to measure the levels of B-cell lymphoma 2 (Bcl-2), caspase-3, and Bcl-2 associated X-protein (Bax) proteins that exist as indicators of apoptosis. Results: EU treatment malondialdehyde (MDA) levels decreased while glutathione (GSH), superoxide dismutase (SOD), A-TOC, and catalase (CAT) activity increased. Following EU therapy dramatically reduced cerebral dysfunction, which was supported by reduced histological damage in I/R injury. Furthermore, with lower levels of MMP-2 and 9, EU treatment could be a novel approach for preventing CI/R injury. EU-treated mice had a notable reduction in cleaved Bax, caspase-3, and increased Bcl-2 protein activities. EU therapy shields the brain against CI/R injury by inhibiting cell death. Conclusion: According to the present study’s findings, EU is a promising neuroprotective agent that can be used to treat CI/R injury in mice. By focusing on these aspects, nurses can provide comprehensive care to patients involved in studies on the effects of EU on CI/R injury, promoting better outcomes through careful monitoring, supportive interventions, and education.

KEYWORDS

• Antiapoptosis
• anti-inflammatory
• eucalyptol
• ischemia–reperfusion
• neuroprotective

Showing references and in-text citations exactly as published.

• DOI10.1177/09731296241297431Copy

RELATED ARTICLES

Matched on section and article type within this issue of Pharmacogn. Mag..

• Article · VOLUME 21, ISSUE 3Bioflavonoid Tangeretin regulates RANK/RANKL/OPG Signaling Proteins via Stimulating Estrogenic Activity in Ovariectomized RatsWeike Tian, Wei Zhang, Li Chen, Junlei Liao, Xuesong Yang · pp. 1001–1012Original Research ArticleSame type: Article
• Article · VOLUME 21, ISSUE 3PhcogMag 21 3 1013Yanhong Zhang, Yanlong He · pp. 1013–1021Original Research ArticleSame type: Article
• Article · VOLUME 21, ISSUE 3Rosmanol Effectively Prevents Diethylnitrosamine-induced Hepatocellular Carcinoma in Rats by Regulating the PI3K/Akt Signaling PathwayWei Cao, Qi Liu, Ying Wang, Lianfeng Chen · pp. 1022–1033Original Research ArticleSame type: Article
• Article · VOLUME 21, ISSUE 3Liquiritin Promotes Vasodilation Effects by Activating the Kv and KATP Channels in the Isolated Rat Thoracic AortaMengXue Ma, YouLan Che, Xin Yang, Zhi Wang, Ya Wen Bai, Yang Yu, QingBin Xu, Ru Zhou · pp. 1034–1042Original Research ArticleSame type: Article