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    Antiproliferative and Apoptotic Potential of Chicoric Acid, a Major Phytoconstituent of Cichorium intybus, against Colon Cancer Cells: A Possible Inhibition of CDC25 Phosphatases

    Modhi O. Alotaibi1, Mahima Verma2, Shireen Fatima2, Nahaa M. Alotaibi1, Nawaf Alshammari3, Mohd Saeed3, F. K. Alharbi4, Irfan Ahmad Ansari2★★ Corresponding author

    1. 1Department of Biology, College of Science, Princess Nourah Bint Abdulrahman University, Riyadh, SAUDI ARABIA.
    2. 2Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, INDIA.
    3. 3Department of Biology, College of Science, University of Hail, Hail, SAUDI ARABIA.
    4. 4Department of Biology, College of Science, Qassim University, Buraydah, SAUDI ARABIA.

    CORRESPONDENCE

    Irfan Ahmad Ansari

    Department of Biosciences, Integral University, Lucknow, Uttar Pradesh, INDIA.

    ahmadirfan.amu@gmail.com

    Received: 20-05-2024; Accepted: 06-09-2024.

    Volume 21, Issue 3 · pp. 984–1000 · PUBLISHED 2025 · DOI: 10.1177/09731296241291625

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Over the years, plants have been utilized worldwide for the treatment of cancer in the traditional system of medicine, notably in the majority of developing nations. The use of chemotherapeutic drugs is frequently associated with lethal side effects when treating cancer. Therefore, the panacea to the vicious side effects linked to synthetic pharmaceuticals is the alternative usage of widely accessible and affordable therapeutic approaches. Chicoric acid, a major constituent of Cichorium intybus, has recently been shown to exhibit antioxidant, anti-inflammatory, antidiabetic, antiviral, neuroprotective, hepatoprotective, and anticancer properties. Objectives: Except for a few preliminary studies, no detailed study on the chemotherapeutic efficacy of chicoric acid against cancer cells has been done till now. Therefore, the aim of this study is to assess the potential of chicoric acid against colon cancer cells. Materials and Methods: Different in vitro assays like MTT, DAPI staining, rhodamine staining, H2DCFDA staining, caspase activity, and cell analysis were performed on colon cancer HCT116 cells to assess their growth inhibitory properties. Results: According to the results, chicoric acid dose-dependently suppressed the growth of colon cancer HCT116 cells by inducing cell cycle arrest at G0/G1 phase and apoptosis via both intrinsic and extrinsic pathways. In silico findings unveiled the cell cycle regulatory capacity of chicoric acid through binding CDC25 phosphatases, implying its potential as an antiproliferative agent capable of suppressing cell growth. Conclusion: Collectively, the results suggested strong chemopreventive potential of chicoric acid against colon cancer cells. Thus, chicoric acid may turn out to be an excellent anticancer agent, which may be explored in the future for the therapeutic drug development against cancer.

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      Alotaibi, M. O., Verma, M., Fatima, S., Alotaibi, N. M., Alshammari, N., Saeed, M., Alharbi, F. K., & Ansari, I. A. (2025). Antiproliferative and Apoptotic Potential of Chicoric Acid, a Major Phytoconstituent of Cichorium intybus, against Colon Cancer Cells: A Possible Inhibition of CDC25 Phosphatases. Pharmacognosy Magazine, 21(3), 984–1000. https://doi.org/10.1177/09731296241291625