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    In vitro tetraploid induction and generation of tetraploids from mixoploids in Dioscorea zingiberensis

    He-Ping Huang1,2,3, Shan-Lin Gao1★, Lan-Lan Chen1, Kun-Hua Wei1★ Corresponding author

    1. 1Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, 211198, China.
    2. 2School of Pharmacy, Anhui University of Traditional Chinese Medicine, Hefei 230031, China.
    3. 3Anhui Key Laboratory of Modernized Chinese Material, Hefei 230031, China.

    CORRESPONDENCE

    Shan-Lin Gao

    Department of Genetics and Breeding, China Pharmaceutical University, Nanjing, 211198, China.

    hhp760222@sohu.com

    Received: 09-12-2009; Revised: 22-12-2009; Accepted: 22-12-2009.

    Volume 6, Issue 21 · pp. 51–56 · PUBLISHED 13 February 2010 · DOI: 10.4103/0973-1296.59966

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    This article describes an efficient colchicine-mediated technique for the in vitro induction of tetraploids in Dioscorea zingiberensis and its confirmation by flow cytometry. Buds immersed in 0.2% colchicine solution for 36 hours prior to culture induced as high as 35.6% tetraploid plants. Colchicine-induced tetraploids remained stable after six months in soil. Leaf characteristics of diploids and tetraploids in D. zingiberensis were compared. It was determined that the leaf sizes of glasshouse-grown plants and stomatal sizes of both in vitro and glasshouse-grown plants were suitable parameters for identifying putative tetraploids in D. zingiberensis. Besides generating tetraploids, this technique generated mixoploids in D. zingiberensis. Calli derived from mixoploid leaves were induced to form buds and shoots. Individual shoots were classed as diploid, mixoploid, and tetraploid by flow cytometry. This callus-based technique could be employed when a genome-doubling agent generated mixoploids, but no tetraploids.

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      Huang, H., Gao, S., Chen, L., & Wei, K. (2010). In vitro tetraploid induction and generation of tetraploids from mixoploids in Dioscorea zingiberensis. Pharmacognosy Magazine, 6(21), 51–56. https://doi.org/10.4103/0973-1296.59966