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    Pharmacognosy MagazineNOW IN
    Article

    Protective effects on myelosuppression mice treated by three different classic Chinese medicine formulae

    An-bin Zhao1★, Bin YU2★, Xian-Lin WU2, Ke-Jian Cao3, En-Qing LI2, Qing-Mei LI4, Xiao-Yin Chen2★★ Corresponding author

    1. 1Department of Internal Medicine, Xinzheng Chinese Medical Hospital, Zhengzhou, People's Republic of China.
    2. 2Department of Traditional Chinese Medicine, Medical College, Jinan University, Guangzhou, People's Republic of China.
    3. 3Department of Traditional Chinese Medicine, School of Chinese Medicine, The University of Hong Kong, Hong Kong, China.
    4. 4Department of Laboratory, Guangzhou Red Cross Hospital, Guangzhou, People's Republic of, China.

    CORRESPONDENCE

    An-bin Zhao

    Department of Internal Medicine, Xinzheng Chinese Medical Hospital, Zhengzhou, People's Republic of China.

    tchenxiaoyin@jnu.edu.cn

    Received: 16-09-2010; Revised: 03-10-2010.

    Volume 7, Issue 26 · pp. 133–140 · PUBLISHED 9 May 2011 · DOI: 10.4103/0973-1296.80671

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: In order to observe the protective therapeutic action and mechanism of Liuwei Dihuang Decoction, Buzhong Yiqi Decoction, and Compound Danshen Decoction on Myelosuppression induced by cyclophosphamide. Materials and Methods: The mice model was established by intraperitoneal injected with 100 mg/kg cyclophosphamide by human and mice dose conversion on the 9th, 11th, 13th days during the experiment. Flow cytometry (FCM) was used for detecting the number of cells and investigating bone marrow cell cycles. Spleen was taken out and the mRNA expression level of thrombopoietin (TPO) and c-Mpl were detected by Q-PCR, and c-Mpl in spleen in order to discuss the mechanism of myelosuppression and the protective effects of traditional Chinese medicine. Results: Both Liuwei Dihuang Decoction Group and Buzhong Yiqi Decoction Group can accelerate bone marrow hematopoietic stem progenitor cells (HSPCs) in marrow-suppressed mice and enhance cell proliferation by promoting cell cycles from G0/G1 phase to access into S, G2/M phase. And at the same time these Chinese decoctions can increase the mRNA expression level of TPO and c-Mpl in spleen. Conclusion: Researched showed that Chinese formula take effect by affecting these genes on myelosuppressed mice.

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      Zhao, A., YU, B., WU, X., Cao, K., LI, E., LI, Q., & Chen, X. (2011). Protective effects on myelosuppression mice treated by three different classic Chinese medicine formulae. Pharmacognosy Magazine, 7(26), 133–140. https://doi.org/10.4103/0973-1296.80671