Validated reversed phase-high performance liquid chromatography-diode array detector method for the quantitation of Rutin, a natural immunostimulant for improving survival in aquaculture practice, in toonea sinensis folium
Yuping Shen1, Huawu Yin1, Bin Chen2, Guohua Xia1, Huan Yang1, Xiaobin Jia2★★ Corresponding author
- 1Department of Chinese Medicine and Pharmacy, School of Pharmacy, Jiangsu University, China.
- 2Jiangsu Provincial Academy of Chinese Medicine, Key Laboratory of New Drug Delivery System of Chinese Materia Medica, China.
CORRESPONDENCE
Xiaobin Jia
Jiangsu Provincial Academy of Chinese Medicine, Key Laboratory of New Drug Delivery System of Chinese Materia Medica, China.
Received: 30-05-2011; Revised: 16-07-2011.
Volume 8, Issue 29 · pp. 49–53 · PUBLISHED 28 February 2012 · DOI: 10.4103/0973-1296.93322
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ABSTRACT
Background: Rutin is a biofl avonoid of strong immunostimulating activity from the Toonea Sinensis Folium, which has shown a signifi cant ability to increase the survival rate of white shrimp with bacterial infection. However, no method for the quantitation of this active ingredient in the herb has been reported to date. Materials and Methods: A reversed phase-high performance liquid chromatography-diode array detector (RP-HPLC-DAD) method was developed to quantify Rutin in the Toonea Sinensis Folium, with the HPLC conditions optimized, followed by validation for linearity, accuracy, precision, limit of detection (LOD), repeatability, and stability. Then, the established method was used to determine the content of Rutin in two samples. Results: The separation was performed on a Waters XBridge Shield RP18 column (150 mm × 4.6 mm, 5 μm) kept at 25°C, and acetonitrile and water containing 0.1% acetate acid (18:82, v / v)-composed mobile phase was constantly driven at 1.0 mL / minute during the analysis. Twenty microliters of sample solution or standard solution were injected into the HPLC system and 254 nm was selected to monitor the separation. A strong linear relationship between the peak area and concentration of Rutin was observed within the range of 0.01044 – 0.2610 mg / mL (r2 = 1.0000). The LOD was 0.03915 μg / mL, and recovery of Rutin was from 97.6 to 99.6%. In addition, the method was also validated to be repeatable, stable, precise, and accurate. Conclusions: An effi cient and reliable RP-HPLC-DAD method was established, which could be used for routine analysis of Rutin in Toonea Sinensis Folium and to assist in the quality control of this herb.
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Shen, Y., Yin, H., Chen, B., Xia, G., Yang, H., & Jia, X. (2012). Validated reversed phase-high performance liquid chromatography-diode array detector method for the quantitation of Rutin, a natural immunostimulant for improving survival in aquaculture practice, in toonea sinensis folium. Pharmacognosy Magazine, 8(29), 49–53. https://doi.org/10.4103/0973-1296.93322