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    Article

    Clonal propagation of Phyllanthus amarus: A hepatoprotector

    Janifer R. Xavier1★, Ramaswamy Gnanam2, Muthiah P. Murugan1, Anju Pappachan3★ Corresponding author

    1. 1Scientist, Division of Biotechnology, Defence Institute of High Altitude Research, Defence Research and Development Organisation (DRDO),C/O 56 APO, Ladakh, Jammu and Kashmir, India.
    2. 2Department of Biotechnology, Center for Plant Molecular Biology and Biotechnology, Tamil Nadu Agricultural University, Coimbatore, Tamil Nadu, India.
    3. 3Molecular Biophysics Unit, Indian Institute of Science, Bangalore, India.

    CORRESPONDENCE

    Janifer R. Xavier

    Scientist, Division of Biotechnology, Defence Institute of High Altitude Research, Defence Research and Development Organisation (DRDO),C/O 56 APO, Ladakh, Jammu and Kashmir, India.

    jennipal@gmail.com

    Received: 14-03-2011; Revised: 14-03-2011.

    Volume 8, Issue 29 · pp. 78–82 · PUBLISHED 28 February 2012 · DOI: 10.4103/0973-1296.93332

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: The micropropagation protocol for Phyllanthus amarus, an important medicinal herb used widely for the treatment of hepatitis in ethnomedicinal systems, was standardized with shoot tip and single node explants. Materials and Methods: The micropropagation was carried out for the hyperproducing ecotype (phyllanthin content 463.828 ppm; hypophyllanthin content: 75.469 ppm) collected from Aanaikatti, Coimbatore, and grown in mist chamber, CPMB, TNAU. For micropropagation studies, the leaves were trimmed off and the shoot tips (6 mm long) and nodal segments (single node) were used for initiation. Results: Shoot tips and single node explants gave a maximum of 6.00 and 7.00 multiple shoots per explant with Benzyl Amino Purine (BAP) (1.0mg/L mg/L). Upon subculturing, a shoot length of around 7 cm with an average of eight internodes per shoot was observed after 20 days in the elongation medium supplemented with BAP (0.2 mg/ Lmg/L) and Indole Acetic Acid (IAA) (2.0 mg/L). Seven to ten adventitious roots developed when the elongated microshoots were cultured in half strength MS medium with Indole Butyric Acid (IBA) (2.0 mg/Lmg/L) and NAA (1.0 mg/L mg/L) in 15–20 days after transfer. The rooted shoots acclimatized successfully to fi eld conditions. Conclusion: A method for successful micropropagation of the valuable medicinal plant was established which will provide a better source for continuous supply of plants for manufacturing drugs.

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      Xavier, J. R., Gnanam, R., Murugan, M. P., & Pappachan, A. (2012). Clonal propagation of Phyllanthus amarus: A hepatoprotector. Pharmacognosy Magazine, 8(29), 78–82. https://doi.org/10.4103/0973-1296.93332