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    Quick comparison of Radix Paeonia Alba, Radix Paeonia Rubra, and Cortex Moutan by high performance liquid chromatography coupled with monolithic columns and their chemical pattern recognition

    He Chunnian2,3★, Peng Yong2,3, Feng Yuxiong4, Peng Bing2,3, Wang Zhe2,3, Xiao Peigen2,3★ Corresponding author

    1. 1Institute of Medicinal Plant Development, Chinese Academy of Medical Science, Peking Union Medical College, 151 Malianwa North Road, 100193, Beijing, China.
    2. 2Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, 100193, Beijing, China.
    3. 3Guangxi Traditional Chinese Medical University, 179 Mingxiu Dong Road, Nanning 530001, P. R, China.

    CORRESPONDENCE

    He Chunnian

    Key Laboratory of Bioactive Substances and Resources Utilization of Chinese Herbal Medicine (Peking Union Medical College), Ministry of Education, 100193, Beijing, China.

    xiaopg@public.bta.net.cn

    Received: 22-08-2011; Revised: 12-10-2011.

    Volume 8, Issue 31 · pp. 98–101 · PUBLISHED 2 August 2012 · DOI: 10.4103/0973-1296.99290

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Radix Paeoniae Alba, Radix Paeoniae Rubra, and Cortex Moutan are important Chinese herbs. Their bioactivities and efficacies are similar. However, they have different superior benefits clinically; so, a comprehensive investigation of the chemical difference is necessary and is of great importance for more reasonable quality assessment and proper clinical application of these three herbal medicines. Objective: To establish a high-performance liquid chromatography (HPLC) fingerprint method for the quality control of Radix Paeonia Alba, Radix Paeonia Rubra, and Cortex Moutan, and to compare their main constituents. Materials and Methods: The separations of Radix Paeoniae Alba, Radix Paeoniae Rubra, and Cortex Moutan was carried out, respectively, through a gradient elution using a monolithic column and a mobile phase consisting of water (containing 0.1% formic acid) and acetonitrile (containing 0.1% formic acid) at a flow rate of 5.0 ml/min. The detection wavelength was set at 230 nm. The data calculation was performed with CHROMAP v1.51 and Statistical Package for the Social Sciences (SPSS) 18.0 software for principal component analysis. Results: A rapid separation method based on high-performance liquid chromatography with diode-array detection (HPLC-DAD) with monolithic columns and a fingerprint analysis method was established. Fifteen Radix Paeonia Alba, 45 Radix Paeonia Rubra, and 21 Cortex Moutan samples were analyzed and 11 chromatographic peaks were identified. Differences of chromatographic peaks among these three herbal medicines in chemical compositions were revealed. Conclusion: The separation and analysis method are fast and simple, which can be used for chemical fingerprint comparison of Radix Paeonia Alba, Radix Paeonia Rubra, and Cortex Moutan. The results for the evaluation of the three medicines could provide experimental evidence for chemical affinity.

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      Chunnian, H., Yong, P., Yuxiong, F., Bing, P., Zhe, W., & Peigen, X. (2012). Quick comparison of Radix Paeonia Alba, Radix Paeonia Rubra, and Cortex Moutan by high performance liquid chromatography coupled with monolithic columns and their chemical pattern recognition. Pharmacognosy Magazine, 8(31), 98–101. https://doi.org/10.4103/0973-1296.99290