Development of a rapid resolution liquid chromatographydiode array detector method for the determination of three compounds in Ziziphora clinopodioides Lam from different origins of Xinjiang
Shuge Tian2,3, Qian Yu4, Dongdong Wang2,3, Halmuart Upur2,3★★ Corresponding author
- 1Xinjiang Key Laboratory of Famous Prescription and Science of Formulas, China.
- 2Department of Chinese Herbal Medicine, College of TCM, Xinjiang Medical University, Urumqi - 830011, Xinjiang, China.
- 3Traditional Chinese Medical Hospital of Changji Hui Nationality Autonomous Prefecture, Changji- 831100, Xinjiang, China.
CORRESPONDENCE
Halmuart Upur
Department of Chinese Herbal Medicine, College of TCM, Xinjiang Medical University, Urumqi - 830011, Xinjiang, China.
Received: 08-11-2011; Revised: 07-12-2011.
Volume 8, Issue 32 · pp. 280–284 · PUBLISHED 22 November 2012 · DOI: 10.4103/0973-1296.103653
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ABSTRACT
Context: As a traditional Uygur medicinal plant, Z. clinopodioides Lam has various uses in Xinjiang. Aims: A reversed-phase rapid resolution liquid chromatography (RP-RRLC) method with diode array detector (DAD) was developed for simultaneous determination of diosmin, linarin, and pulegone from Ziziphora clinopodioides Lam, a widely used in traditional Uygur medicine for treating heart disease, high blood pressure, and other diseases. Settings and Design: Compounds were separated on a XDB-C18 reversed-phase analytical column (50 mm × 4.6 mm, 1.8 µm) with gradient elution using methanol and 1% aqueous acetic acid (v/v) at 0.9 mL/min. he detection wavelength was set at 270 nm. Materials and Methods: Ziziphora clinopodioides Lam. were collected from ten different origins in Xinjiang, including the Ban fang ditch, Tuoli, the Altay mountains, Terks, Xiata Road, Zhaosu Highway, Guozigou, Fukang, Jimsar, Wulabo. Statistical Analysis Used: The intra-day and inter-day precisions of all three compounds were less than 0.89% and the average recoveries ranged from 97.4 to 104.1%. There were highly significant linear correlations between component concentrations and specific chromatographic peak areas (R2 > 0.999). Results: The proposed method was successfully applied to determine the levels of three active components in Z. clinopodioides Lam. samples from different locations in Xinjiang. Conclusions: The proposed method is simple, consistent, accurate, and could be utilized as a quality control method for Z. clinopodioides Lam.
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Tian, S., Yu, Q., Wang, D., & Upur, H. (2012). Development of a rapid resolution liquid chromatographydiode array detector method for the determination of three compounds in Ziziphora clinopodioides Lam from different origins of Xinjiang. Pharmacognosy Magazine, 8(32), 280–284. https://doi.org/10.4103/0973-1296.103653