Phcog.Net logo
Submit

BROWSE ALL JOURNALS

    SEE ALL 6 JOURNALS
    Pharmacognosy MagazineNOW IN
    Article

    Qualitative and quantitative analysis of nine major compounds in the Bozhougyiqi-Tang using a highperformance liquid chromatography coupled with a diode array detector and electrospray ionization mass spectrometer

    Jin Bae Weon2★, Jin Yeul Ma3, Hye Jin Yang2, Bohyoung Lee2, Bo-Ra Yun2, Choong Je Ma2,4★ Corresponding author

    1. 1Department of Medical Biomaterials Engineering, College of Biomedical Science, Kangwon National University, Chuncheon 200-701.
    2. 2TKM Converging Research Division, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong-gu, Daejeon 305-811, Korea.
    3. 3Research Institute of Biotechnology, Kangwon National University, Chuncheon 200-701.

    CORRESPONDENCE

    Jin Bae Weon

    TKM Converging Research Division, Korea Institute of Oriental Medicine, 483 Exporo, Yuseong-gu, Daejeon 305-811, Korea.

    Received: 29-06-2012; Revised: 28-07-2012.

    Volume 9, Issue 35 · pp. 271–282 · PUBLISHED 11 June 2013 · DOI: 10.4103/0973-1296.113291

    View on Pharmacogn. Mag. original site ↗

    ABSTRACT

    Background: Bozhougyiqi-Tang (BZYQT) is of traditional herbal medicine used for enhancement of digestive capacity. Objective: An accurate and reliable simultaneous determination using a HPLC-DAD and ESI-MS was developed and validated for the qualitative and quantitative analysis of 9 major compounds, ferulic acid (1), naringin (2), hesperidin (3), decursinol (4), glycyrrhizin (5), saikosaponin A (6), 6-gingerol (7), ginsenoside Rg3 (8), decursin (9), in traditional herbal medicine ‘Bozhougyiqi-Tang.’ Materials and Methods: The chromatographic separation of 9 compounds was performed on a SHISEIDO C18 column (250 mm × 4.6 mm i.d., S-5 μm) using gradient elution with 0.1% trifluoroacetic acid and acetonitrile at a flow rate of 1.0 ml/min. The 9 compounds were identified based on peak retention time and UV spectrum and MS data of these compounds. Results: This developed method showed good linearity (R2 > 0.999). The LOD and LOQ of the major compounds were less than 0.09 and 0.28 μg/ml, respectively. The intra - day and inter - day RSD values were within 2.06% and 1.64%, respectively. The mean recoveries were from 92.10% to 108.56% with less than 1.88%. The results indicated that established method had good precision and accuracy. Conclusion: The new method was successfully applied to the simultaneous analysis of 9 compounds in Bozhougyiqi-Tangs samples.

    KEYWORDS

    Open in new tab

    REFERENCES

    As published

    Showing references and in-text citations exactly as published.

      Cite this article

      SELECT FORMAT

      Weon, J. B., Ma, J. Y., Yang, H. J., Lee, B., Yun, B., & Ma, C. J. (2013). Qualitative and quantitative analysis of nine major compounds in the Bozhougyiqi-Tang using a highperformance liquid chromatography coupled with a diode array detector and electrospray ionization mass spectrometer. Pharmacognosy Magazine, 9(35), 271–282. https://doi.org/10.4103/0973-1296.113291